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Product Info Summary
| SKU: | A06354 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DGK- alpha Antibody
SKU/Catalog Number
A06354
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DGK- alpha Antibody catalog # A06354. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DGK- alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06354)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DGKA. AA range:304-353
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06354 is reactive to DGKA in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
82630 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06354 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of LOVO cells using DGK-alpha Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using DGK-alpha Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For DGKA (Source: Uniprot.org, NCBI)
Gene Name
DGKA
Full Name
Diacylglycerol kinase alpha
Weight
82630 MW
Superfamily
eukaryotic diacylglycerol kinase family
Alternative Names
Diacylglycerol kinase alpha;DAG kinase alpha;2.7.1.107;80 kDa diacylglycerol kinase;Diglyceride kinase alpha;DGK-alpha;DGKA;DAGK, DAGK1; DGKA DAGK, DAGK1, DGK-alpha diacylglycerol kinase alpha diacylglycerol kinase alpha|80 kDa diacylglycerol kinase|DAG kinase alpha|diacylglycerol kinase, alpha 80kDa|diglyceride kinase alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DGKA, check out the DGKA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DGKA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DGK- alpha Antibody (A06354)
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No publications found for A06354
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Customer Q&As
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6 Customer Q&As for Anti-DGK- alpha Antibody
Question
I see that the anti-DGK- alpha antibody A06354 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-09
Question
Would anti-DGK- alpha antibody A06354 work on feline WB with uterus?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
Our lab technicians have not validated anti-DGK- alpha antibody A06354 on feline. You can run a BLAST between feline and the immunogen sequence of anti-DGK- alpha antibody A06354 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-DGK- alpha antibody A06354. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-08
Question
I was wanting to use your anti-DGK- alpha antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
S. Jones
Verified customer
Asked: 2019-06-26
Answer
It shows on the product datasheet, A06354 anti-DGK- alpha antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-26
Question
Would A06354 anti-DGK- alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
It shows on the product datasheet, A06354 anti-DGK- alpha antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-10
Question
We are currently using anti-DGK- alpha antibody A06354 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
A. Baker
Verified customer
Asked: 2015-11-30
Answer
The anti-DGK- alpha antibody (A06354) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-30
