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Product Info Summary
| SKU: | M01948-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Desmin Rabbit Monoclonal Antibody
SKU/Catalog Number
M01948-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Desmin Rabbit Monoclonal Antibody catalog # M01948-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Desmin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01948-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BDF-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Desmin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01948-1 is reactive to DES in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
72 kDa
Calculated molecular weight
53536 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01948-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:10000-1:20000
IHC 1:100-1:500
ICC/IF 1:50-1:100
Positive Control
IHC: human prostate tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human prostate, using Desmin Antibody.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For DES (Source: Uniprot.org, NCBI)
Gene Name
DES
Full Name
Desmin
Weight
53536 MW
Superfamily
intermediate filament family
Alternative Names
Desmin;DES; DES CDCD3, CSM1, CSM2, LGMD1D, LGMD1E, LGMD2R desmin desmin|cardiomyopathy, dilated 1F (autosomal dominant)|epididymis secretory sperm binding protein|intermediate filament protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DES, check out the DES Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DES: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Desmin Rabbit Monoclonal Antibody (M01948-1)
Hello CJ!
M01948-1 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Identification of apoptosis-related microRNAs and their target genes in myocardial infarction post-transplantation with skeletal myoblasts
Liu J, Wang Y, Du W, Liu W, Liu F, Zhang L, Zhang M, Hou M, Liu K, Zhang S, Yu B. Plos One. 2013;8(3):E58883. Doi: 10.1371/Journal.Pone.0058883. Epub 2013 Mar 22. Wnt1 Inhibits Hydrogen Peroxide-Induced Apoptosis In Mouse Cardiac Stem Cells.
Pan X, Chen Z, Huang R, Yao Y, Ma G. Plos One. 2013;8(4):E60335. Doi: 10.1371/Journal.Pone.0060335. Epub 2013 Apr 1. Transforming Growth Factor ??1 Induces The Expression Of Collagen Type I By Dna Methylation In Cardiac Fibroblasts.
Qian Q, Qian H, Zhang X, Zhu W, Yan Y, Ye S, Peng X, Li W, Xu Z, Sun L, Xu W. Stem Cells Dev. 2012 Jan;21(1):67-75. Doi: 10.1089/Scd.2010.0519. Epub 2011 Jun 1. 5-Azacytidine Induces Cardiac Differentiation Of Human Umbilical Cord-Derived Mesenchy...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Desmin Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-Desmin Rabbit Monoclonal antibody for WB for rat muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat muscle identification?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
As indicated on the product datasheet, M01948-1 anti-Desmin Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-20
Question
Is this M01948-1 anti-Desmin Rabbit Monoclonal antibody reactive to the isotypes of DES?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The immunogen of M01948-1 anti-Desmin Rabbit Monoclonal antibody is A synthesized peptide derived from human Desmin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-12
Question
you antibody to test anti-Desmin Rabbit Monoclonal antibody M01948-1 on rat muscle for research purposes, then I may be interested in using anti-Desmin Rabbit Monoclonal antibody M01948-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
The products we sell, including anti-Desmin Rabbit Monoclonal antibody M01948-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle using anti-Desmin Rabbit Monoclonal antibody M01948-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-08
Question
Is a blocking peptide available for product anti-Desmin Rabbit Monoclonal antibody (M01948-1)?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
We do provide the blocking peptide for product anti-Desmin Rabbit Monoclonal antibody (M01948-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-09
Question
I see that the anti-Desmin Rabbit Monoclonal antibody M01948-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-05
Question
My question regarding product M01948-1, anti-Desmin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01948-1 anti-Desmin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-26
Question
We have observed staining in mouse liver. Any tips? Is anti-Desmin Rabbit Monoclonal antibody supposed to stain liver positively?
P. Collins
Verified customer
Asked: 2019-04-10
Answer
From what I have seen in literature liver does express DES. From what I have seen in Uniprot.org, DES is expressed in esophagogastric junction muscularis propria, muscle, skeletal muscle, liver, among other tissues. Regarding which tissues have DES expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 8792816, 15489334
Skeletal muscle, Pubmed ID: 10545598
Boster Scientific Support
Answered: 2019-04-10
Question
Will M01948-1 anti-Desmin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
As indicated on the product datasheet, M01948-1 anti-Desmin Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-26
Question
Will anti-Desmin Rabbit Monoclonal antibody M01948-1 work for WB with muscle?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
According to the expression profile of muscle, DES is highly expressed in muscle. So, it is likely that anti-Desmin Rabbit Monoclonal antibody M01948-1 will work for WB with muscle.
Boster Scientific Support
Answered: 2018-10-22
Question
Is there a BSA free version of anti-Desmin Rabbit Monoclonal antibody M01948-1 available?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Desmin Rabbit Monoclonal antibody M01948-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-19
Question
I would like using your anti-Desmin Rabbit Monoclonal antibody for myopathy studies. Has this antibody been tested with western blotting on mouse heart tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
We appreciate your inquiry. This M01948-1 anti-Desmin Rabbit Monoclonal antibody is tested on tissue lysate, heart tissue, mouse heart tissue, rat heart tissue. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-10-13
Question
Our team were content with the WB result of your anti-Desmin Rabbit Monoclonal antibody. However we have been able to see positive staining in liver sarcomere using this antibody. Is that expected? Could you tell me where is DES supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-05-16
Answer
Based on literature, liver does express DES. Generally DES expresses in cytoplasm, myofibril, sarcomere, z line. Regarding which tissues have DES expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 8792816, 15489334
Skeletal muscle, Pubmed ID: 10545598
Boster Scientific Support
Answered: 2017-05-16
Question
See below the WB image, lot number and protocol we used for muscle using anti-Desmin Rabbit Monoclonal antibody M01948-1. Please let me know if you require anything else.
M. Huang
Verified customer
Asked: 2016-03-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-25
Question
We are currently using anti-Desmin Rabbit Monoclonal antibody M01948-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
A. Carter
Verified customer
Asked: 2015-10-13
Answer
The anti-Desmin Rabbit Monoclonal antibody (M01948-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-10-13
Question
We ordered your anti-Desmin Rabbit Monoclonal antibody for IF on liver a few months ago. I am using rat, and We intend to use the antibody for IHC next. I am interested in examining liver as well as muscle in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
D. Miller
Verified customer
Asked: 2015-04-02
Answer
I have checked the website and datasheets of our anti-Desmin Rabbit Monoclonal antibody and it appears that M01948-1 has been tested on rat in both IF and IHC. Thus M01948-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-04-02
