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Product Info Summary
| SKU: | M05297 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-delta Sarcoglycan SGCD Rabbit Monoclonal Antibody
View all delta-Sarcoglycan Antibodies
SKU/Catalog Number
M05297
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-delta Sarcoglycan SGCD Rabbit Monoclonal Antibody catalog # M05297. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-delta Sarcoglycan SGCD Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05297)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AbS97
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human delta Sarcoglycan
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05297 is reactive to SGCD in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
32 kDa
Calculated molecular weight
32071 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05297 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: Human fetal heart
IHC: human stomach carcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of delta Sarcoglycan expression in Human fetal heart lysate.
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Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-delta Sarcoglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Protein Target Info & Infographic
Gene/Protein Information For SGCD (Source: Uniprot.org, NCBI)
Gene Name
SGCD
Full Name
Delta-sarcoglycan
Weight
32071 MW
Superfamily
sarcoglycan beta/delta/gamma/zeta family
Alternative Names
Delta-sarcoglycan;Delta-SG;35 kDa dystrophin-associated glycoprotein;35DAG;SGCD; SGCD 35DAG, CMD1L, DAGD, LGMDR6, SG-deltaP, SGD, SGCD sarcoglycan delta delta-sarcoglycan|35 kDa dystrophin-associated glycoprotein|delta-SG|dystrophin associated glycoprotein, delta sarcoglycan|placental delta sarcoglycan|sarcoglycan, delta (35kDa dystrophin-associated glycoprotein)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SGCD, check out the SGCD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SGCD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-delta Sarcoglycan SGCD Rabbit Monoclonal Antibody (M05297)
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No publications found for M05297
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Customer Q&As
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6 Customer Q&As for Anti-delta Sarcoglycan SGCD Rabbit Monoclonal Antibody
Question
Here is the WB image, lot number and protocol we used for skeletal muscle using anti-delta Sarcoglycan Rabbit Monoclonal antibody M05297. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-22
Question
We are currently using anti-delta Sarcoglycan Rabbit Monoclonal antibody M05297 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-delta Sarcoglycan Rabbit Monoclonal antibody (M05297) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-08
Question
Do you have a BSA free version of anti-delta Sarcoglycan Rabbit Monoclonal antibody M05297 available?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-delta Sarcoglycan Rabbit Monoclonal antibody M05297 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-12
Question
Is a blocking peptide available for product anti-delta Sarcoglycan Rabbit Monoclonal antibody (M05297)?
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
We do provide the blocking peptide for product anti-delta Sarcoglycan Rabbit Monoclonal antibody (M05297). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-27
Question
I see that the anti-delta Sarcoglycan Rabbit Monoclonal antibody M05297 works with WB, what is the protocol used to produce the result images on the product page?
R. Taylor
Verified customer
Asked: 2018-03-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-19
Question
Will anti-delta Sarcoglycan Rabbit Monoclonal antibody M05297 work on pig IP with lung?
A. Zhao
Verified customer
Asked: 2015-03-18
Answer
Our lab technicians have not validated anti-delta Sarcoglycan Rabbit Monoclonal antibody M05297 on pig. You can run a BLAST between pig and the immunogen sequence of anti-delta Sarcoglycan Rabbit Monoclonal antibody M05297 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig lung in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-18
