Anti-DDX58 Monoclonal Antibody

Product Name

Anti-DDX58 Monoclonal Antibody

View all RIG-I Antibodies

SKU/Catalog Number

M00244

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-DDX58 Monoclonal Antibody catalog # M00244. Tested in WB, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DDX58 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00244)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACEA-4

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human DDX58 Retinoic acid inducible gene I (RIG-I) is a 925 amino acid, interferon-inducible cellular DExD/H box RNA helicase that activates type I interferon (IFN), an important effector of the innate immune system that is sensitive to these dsRNA viruses. dsRNA is normally present in very low quantities in cells, so when a virus is present, the elevated levels of dsRNA act as a sign telling RIG-I to activate the production of IFN.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00244 is reactive to DDX58 in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

100-110 kDa

Calculated molecular weight

15145 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00244 is guaranteed for IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IP 1:50

Positive Control

WB: Jurkat cell

Validation Images & Assay Conditions

Gene/Protein Information For DDX58 (Source: Uniprot.org, NCBI)

Alternative Names

Histone H2AX;H2a/x;Histone H2A.X;H2AFX;H2AX; DDX58 RIG-I, RIG1, RIGI, RLR-1, SGMRT2 DExD/H-box helicase 58 antiviral innate immune response receptor RIG-I|DEAD (Asp-Glu-Ala-Asp) box polypeptide 58|DEAD box protein 58|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|RNA helicase RIG-I|probable ATP-dependent RNA helicase DDX58|retinoic acid-inducible gene 1 protein|retinoic acid-inducible gene I protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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