Anti-Daxx Antibody

Product Name

Anti-Daxx Antibody

View all DAXX Antibodies

SKU/Catalog Number

A00439

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Daxx Antibody (Catalog # A00439). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Daxx antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Daxx Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00439)

Host

Rabbit

Contents

Daxx Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Daxx antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human Daxx. The immunogen is located within the last 50 amino acids of Daxx.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

ALSFTD antibody is human, mouse and rat reactive. At least two isoforms are known to exist.

Reactive Species

A00439 is reactive to DAXX in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

81373 MW

Background of DAXX

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx. Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00439 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Daxx antibody can be used for detection of Daxx by Western blot at 1 μg/mL. A 120 kDa major band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For DAXX (Source: Uniprot.org, NCBI)

Alternative Names

DAP6, EAP1, BING2, DAP6, Death domain-associated protein 6, Daxx, hDaxx DAXX BING2, DAP6, EAP1, SMIM40 death domain associated protein death domain-associated protein 6|CENP-C binding protein|ETS1-associated protein 1|Fas-binding protein|death-associated protein 6|fas death domain-associated protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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