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Product Info Summary
| SKU: | A10097 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Dapper homolog 2 Dact2 Antibody
SKU/Catalog Number
A10097
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Dapper homolog 2 Dact2 Antibody (Catalog # A10097). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Dact2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Dapper homolog 2 Dact2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10097)
Host
Rabbit
Contents
Dact2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Dact2 antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human DACT2. The immunogen is located within amino acids 150 - 200 of Dact2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with MANF.
Reactive Species
A10097 is reactive to DACT2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
82700 MW
Background of DACT2
The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact2, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact2 is most prominent during the development of the thymus kidneys, and salivary gland. Dact2 is thought to play a role distinct from that of Dact1 with Dact2 having a greater impact on a beta-catenin-independent process termed planar cell polarity/convergent-extension signaling. Furthermore, Dact2 but not Dact1 can inhibit Nodal signaling by promoting the endocytic degradation of TGF-beta receptors. At least two isoforms of Dact2 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10097 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Dact2 antibody can be used for detection of Dact2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Dact2 in SK-N-SH cell lysate with Dact2 antibody at 1 μg/mL.
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Immunohistochemistry of Dact2 in human brain tissue with Dact2 antibody at 2.5 μg/mL.
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Immunofluorescence of Dact2 in Human Brain cells with Dact2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For DACT2 (Source: Uniprot.org, NCBI)
Gene Name
DACT2
Full Name
Dapper homolog 2
Weight
82700 MW
Superfamily
dapper family
Alternative Names
DPR2, DAPPER2, C6orf116, bA503C24.7, PP13671, Dapper homolog 2, Dapper antagonist of catenin 2 DACT2 C6orf116, DAPPER2, DPR2, bA503C24.7 dishevelled binding antagonist of beta catenin 2 dapper homolog 2|dapper antagonist of catenin 2|dapper homolog 2, antagonist of beta-catenin|dapper, antagonist of beta-catenin, homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DACT2, check out the DACT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DACT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dapper homolog 2 Dact2 Antibody (A10097)
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