Anti-DAPK2 Antibody

Product Name

Anti-DAPK2 Antibody

View all DAP Kinase 2 Antibodies

SKU/Catalog Number

A02241

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-DAPK2 Antibody (Catalog # A02241). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

DAPK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-DAPK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02241)

Host

Rabbit

Contents

DAPK2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DAPK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2. This antigenic peptide is identical to the corresponding amino acids of mouse origin (1,2). The immunogen is located within the last 50 amino acids of DAPK2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

DAPK2 has no cross responses to DAPK1.

Reactive Species

A02241 is reactive to DAPK2 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

42898 MW

Background of DAP Kinase 2

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNγ induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A02241 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DAPK2 antibody can be used for detection of DAPK2 by Western blot at 1 μg/mL. An approximately 42 kDa band can be detected. DAPK2 has no cross responses to DAPK1. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For DAPK2 (Source: Uniprot.org, NCBI)

Alternative Names

DRP1, DRP-1, Death-associated protein kinase 2, DAP-kinase-related protein 1, DAP kinase 2 Dapk2|death-associated protein kinase 2|death-associated protein kinase 2|DAP kinase 2|DRP-1|death-associated kinase 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis