Click image to see more details
Product Info Summary
| SKU: | A00910 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-DAF CD55 Antibody
SKU/Catalog Number
A00910
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DAF CD55 Antibody (Catalog # A00910). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
DAF antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-DAF CD55 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00910)
Host
Rabbit
Contents
DAF antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF. The immunogen is located within amino acids 360 - 410 of DAF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform.
Reactive Species
A00910 is reactive to CD55 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
41400 MW
Background of CD55/DAF
The decay-accelerating factor (DAF), also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1). DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2). Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00910 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DAF body can be used for detection of DAF by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 μg/ml.
Click image to see more details
Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 μg/ml.
Click image to see more details
Immunofluorescence of DAF in human lung tissue with DAF antibody at 20 μg/ml.
Click image to see more details
Western blot analysis of DAF1 expression in HeLa cell lysate with DAF1 antibody at (A) 1 and (B) 2 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For CD55 (Source: Uniprot.org, NCBI)
Gene Name
CD55
Full Name
Complement decay-accelerating factor
Weight
41400 MW
Superfamily
receptors of complement activation (RCA) family
Alternative Names
Decay-accelerating factor, CD55 molecule decay accelerating factor for complement (Cromer blood group), CD55, CR, TC, CROM CD55 CHAPLE, CR, CROM, DAF, TC CD55 molecule (Cromer blood group) complement decay-accelerating factor|CD55 |CD55 molecule, decay accelerating factor for complement (Cromer blood group)|Cromer blood group |Rh blood group D
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD55, check out the CD55 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD55: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DAF CD55 Antibody (A00910)
Hello CJ!
No publications found for A00910
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-DAF CD55 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-DAF CD55 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
