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Product Info Summary
| SKU: | A06054 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-DAD1 Antibody
SKU/Catalog Number
A06054
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DAD1 Antibody (Catalog # A06054). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
DAD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-DAD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06054)
Host
Rabbit
Contents
DAD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1. The immunogen is located within the last 50 amino acids of DAD1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
CRIM1 antibody is human reactive. CRIM1 antibody is predicted to not cross-react with CRIM2.
Reactive Species
A06054 is reactive to DAD1 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
12497 MW
Background of DAD1
Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06054 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μg/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5
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Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.
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Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For DAD1 (Source: Uniprot.org, NCBI)
Gene Name
DAD1
Full Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Weight
12497 MW
Superfamily
DAD/OST2 family
Alternative Names
OST2, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Defender against cell death 1, Oligosaccharyl transferase subunit DAD1 DAD1 OST2 defender against cell death 1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1|DAD-1|oligosaccharyl transferase subunit DAD1|oligosaccharyltransferase 2 homolog|oligosaccharyltransferase subunit 2 (non-catalytic)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DAD1, check out the DAD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DAD1 Antibody (A06054)
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