Anti-Cytokeratin 10 (Suprabasal Epithelial Marker) Monoclonal Antibody

Product Name

Anti-Cytokeratin 10 (Suprabasal Epithelial Marker) Monoclonal Antibody

View all KRT10 Antibodies

SKU/Catalog Number

M02305

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Cytokeratin 10 (Suprabasal Epithelial Marker) Monoclonal Antibody (Catalog # M02305). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Dog, Cat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Cytokeratin 10 (Suprabasal Epithelial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02305)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM623

Isotype

IgG1, kappa

Immunogen

Cytoskeletal preparation extracted from human ectocervical epithelium

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02305 is reactive to KRT10 in Dog, Human, Cat

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

58827 MW

Background of KRT10

This monoclonal antibody recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02305 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For KRT10 (Source: Uniprot.org, NCBI)

Alternative Names

Keratin, type I cytoskeletal 10;Cytokeratin-10;CK-10;Keratin-10;K10;KRT10;KPP; KRT10 BCIE, BIE, CK10, EHK, K10, KPP keratin 10 keratin, type I cytoskeletal 10|CK-10|cytokeratin 10|keratin 10, type I

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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