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Product Info Summary
| SKU: | M00598 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal Antibody
View all Cytochrome P450 1A2 Antibodies
SKU/Catalog Number
M00598
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal Antibody catalog # M00598. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00598)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFAI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytochrome P450 1A2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00598 is reactive to CYP1A2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
32-42 kDa, 65 kDa, 75 kDa
Calculated molecular weight
88279 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00598 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:500
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Cytochrome P450 1A2 expression in Caco2 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CYP1A2 (Source: Uniprot.org, NCBI)
Gene Name
CYP1A2
Full Name
Cytochrome P450 1A2
Weight
88279 MW
Superfamily
cytochrome P450 family
Alternative Names
Dipeptidyl peptidase 4;3.4.14.5 ;ADABP;Adenosine deaminase complexing protein 2;ADCP-2;Dipeptidyl peptidase IV;DPP IV;T-cell activation antigen CD26;TP103;CD26;Dipeptidyl peptidase 4 membrane form;Dipeptidyl peptidase IV membrane form;Dipeptidyl peptidase 4 soluble form;Dipeptidyl peptidase IV soluble form;DPP4;ADCP2, CD26; CYP1A2 CP12, CYPIA2, P3-450, P450(PA) cytochrome P450 family 1 subfamily A member 2 cytochrome P450 1A2|P450 form 4|aryl hydrocarbon hydroxylase|cholesterol 25-hydroxylase|cytochrome P(3)450|cytochrome P450 4|cytochrome P450, family 1, subfamily A, polypeptide 2|cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2|cytochrome P450-P3|dioxin-inducible P3-450|flavoprotein-linked monooxygenase|hydroperoxy icosatetraenoate dehydratase|microsomal monooxygenase|xenobiotic monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP1A2, check out the CYP1A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP1A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal Antibody (M00598)
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No publications found for M00598
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Customer Q&As
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7 Customer Q&As for Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal Antibody
Question
Is this M00598 anti-Cytochrome P450 1A2 Monoclonal antibody reactive to the isotypes of CYP1A2?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
The immunogen of M00598 anti-Cytochrome P450 1A2 Monoclonal antibody is A synthesized peptide derived from human Cytochrome P450 1A2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-28
Question
Is a blocking peptide available for product anti-Cytochrome P450 1A2 Monoclonal antibody (M00598)?
N. Wu
Verified customer
Asked: 2020-04-22
Answer
We do provide the blocking peptide for product anti-Cytochrome P450 1A2 Monoclonal antibody (M00598). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-22
Question
Does anti-Cytochrome P450 1A2 Monoclonal antibody M00598 work on pig WB with liver?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
Our lab technicians have not tested anti-Cytochrome P450 1A2 Monoclonal antibody M00598 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Cytochrome P450 1A2 Monoclonal antibody M00598 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-06
Question
I see that the anti-Cytochrome P450 1A2 Monoclonal antibody M00598 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-12
Question
I was wanting to use your anti-Cytochrome P450 1A2 Monoclonal antibody for ICC for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
It shows on the product datasheet, M00598 anti-Cytochrome P450 1A2 Monoclonal antibody has been tested for Flow Cytometry, IF, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-20
Question
I have a question about product M00598, anti-Cytochrome P450 1A2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00598 anti-Cytochrome P450 1A2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-01
Question
We are interested in to test anti-Cytochrome P450 1A2 Monoclonal antibody M00598 on human liver for research purposes, then I may be interested in using anti-Cytochrome P450 1A2 Monoclonal antibody M00598 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
The products we sell, including anti-Cytochrome P450 1A2 Monoclonal antibody M00598, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-01
