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Product Info Summary
| SKU: | M03529 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Dog, Drosophila, Human, Mouse, Rat, Horse, Frog, Pigeon |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Cytochrome C (Mitochondrial Marker) CYCS Monoclonal Antibody
View all Cytochrome c Antibodies
SKU/Catalog Number
M03529
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Cytochrome C (Mitochondrial Marker) CYCS Monoclonal Antibody (Catalog # M03529). Tested in WB, Flow Cytometry, IHC applications. This antibody reacts with Human, Mouse, Rat, Horse, Dog, Pigeon, Frog, Drosophila.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Cytochrome C (Mitochondrial Marker) CYCS Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03529)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: CTC05
Isotype
IgG2b, kappa
Immunogen
Recombinant cytochrome c protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M03529 is reactive to CYCS in Dog, Drosophila, Human, Mouse, Rat, Horse, Frog, Pigeon
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
11749 MW
Background of Cytochrome c
Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03529 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Heart stained with Anti-Cytochrome C Monoclonal Antibody (CTC05).
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Western Blot analysis of heart tissue lysate using Anti-Cytochrome C Mouse Monoclonal Antibody (CTC05).
Protein Target Info & Infographic
Gene/Protein Information For CYCS (Source: Uniprot.org, NCBI)
Gene Name
CYCS
Full Name
Cytochrome c, somatic
Weight
11749 MW
Superfamily
cytochrome c family
Alternative Names
Cytochrome c;CYCS;CYC; Cycs|cytochrome c, somatic|cytochrome c, somatic
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYCS, check out the CYCS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYCS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome C (Mitochondrial Marker) CYCS Monoclonal Antibody (M03529)
Hello CJ!
M03529 has been cited in 13 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang T,Chen Y,Cai J,Pan M,Sun Q,Zhang J,Sun C.SOCS2 Inhibits Mitochondrial Fatty Acid Oxidation via Suppressing LepR/JAK2/AMPK Signaling Pathway in Mouse Adipocytes.Oxid Med Cell Longev.2020 Jul 13;2020:3742542.doi:10.1155/2020/3742542.PMID:32733634;PMCI
Species: Mouse
Pioglitazone improves mitochondrial function in the remnant kidney and protects against renal fibrosis in 5/6 nephrectomized rats
Neuroprotective Effect of Tea Polyphenols on Oxyhemoglobin Induced Subarachnoid Hemorrhage in Mice
pH-sensitive micelles for the intracellular co-delivery of curcumin and Pluronic L61 unimers for synergistic reversal effect of multidrug resistance
Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells
Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway
Methacryloxylethyl Cetyl Ammonium Chloride Induces DNA Damage and Apoptosis in Human Dental Pulp Cells via Generation of Oxidative Stress
N-Acetyl Cysteine Depletes Reactive Oxygen Species and Prevents Dental Monomer-Induced Intrinsic Mitochondrial Apoptosis In Vitro in Human Dental Pulp Cells
FABP4 reversed the regulation of leptin on mitochondrial fatty acid oxidation in mice adipocytes
Guo X, Fu Y, Wang Z, Wang T, Li C, Huang T, Gao F, Li C. Oxid Med Cell Longev. 2018 Mar 25;2018:4950705. doi: 10.1155/2018/4950705. eCollection 2018. Di-2-pyridylhydrazone Dithiocarbamate Butyric Acid Ester Exerted Its Proliferative Inhibition aga...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Cytochrome C (Mitochondrial Marker) CYCS Monoclonal Antibody
Question
Would anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529 work on feline IHC with lung?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
Our lab technicians have not validated anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline lung in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-25
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-15
Question
We have been able to see staining in human cerebellum. What should we do? Is anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody supposed to stain cerebellum positively?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
From what I have seen in literature cerebellum does express CYCS. From what I have seen in Uniprot.org, CYCS is expressed in heart, cerebellum, amygdala, bone marrow, brain, kidney, lung, skeletal muscle, skin,
rc testis urinary bladder, erythroleukemia, liver, among other tissues. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-08-30
Question
Will M03529 anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
As indicated on the product datasheet, M03529 anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-30
Question
Is a blocking peptide available for product anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody (M03529)?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We do provide the blocking peptide for product anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody (M03529). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-26
Question
I was wanting to use your anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody for IHC for canine heart on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for canine heart identification?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
As indicated on the product datasheet, M03529 anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody has been validated for IHC on canine, equine, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in canine heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-20
Question
Is this M03529 anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody reactive to the isotypes of CYCS?
S. Gonzalez
Verified customer
Asked: 2019-04-12
Answer
The immunogen of M03529 anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody is Recombinant cytochrome c protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-12
Question
I see that the anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-17
Question
Do you have a BSA free version of anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529 available?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-13
Question
See below the WB image, lot number and protocol we used for heart using anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-10
Question
I would like to test anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529 on canine heart for research purposes, then I may be interested in using anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-29
Answer
The products we sell, including anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-29
Question
My boss were satisfied with the WB result of your anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody. However we have seen positive staining in heart mitochondrion intermembrane space. using this antibody. Is that expected? Could you tell me where is CYCS supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
From literature, heart does express CYCS. Generally CYCS expresses in mitochondrion intermembrane space. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2017-11-01
Question
Can you help my question with product M03529, anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03529 anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-07
Question
Does anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529 work for IHC with heart?
E. Zhang
Verified customer
Asked: 2017-06-28
Answer
According to the expression profile of heart, CYCS is highly expressed in heart. So, it is likely that anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529 will work for IHC with heart.
Boster Scientific Support
Answered: 2017-06-28
Question
We are currently using anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody M03529 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says canine, equine, human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
K. Baker
Verified customer
Asked: 2015-02-27
Answer
The anti-Cytochrome C (Mitochondrial Marker) Monoclonal antibody (M03529) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-02-27
