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Product Info Summary
| SKU: | A16342 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Cytochrome b561 D1 CYB561D1 Antibody
SKU/Catalog Number
A16342
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cytochrome b561 D1 CYB561D1 Antibody (Catalog# A16342). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome b561 D1 CYB561D1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16342)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CYB561D1. Immunogen sequence location: 180-229
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A16342 is reactive to CYB561D1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
25424 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16342 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from HeLa cells and COLO205 cells, using Cytochrome b561 D1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of MCF-7 cells, using Cytochrome b561 D1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CYB561D1 (Source: Uniprot.org, NCBI)
Gene Name
CYB561D1
Full Name
Cytochrome b561 domain-containing protein 1
Weight
25424 MW
Alternative Names
CYB561D1;Cytochrome b561 domain-containing protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYB561D1, check out the CYB561D1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYB561D1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome b561 D1 CYB561D1 Antibody (A16342)
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No publications found for A16342
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Customer Q&As
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6 Customer Q&As for Anti-Cytochrome b561 D1 CYB561D1 Antibody
Question
My question regarding product A16342, anti-Cytochrome b561 D1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A16342 anti-Cytochrome b561 D1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-19
Question
We are currently using anti-Cytochrome b561 D1 antibody A16342 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
The anti-Cytochrome b561 D1 antibody (A16342) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-04
Question
See below the WB image, lot number and protocol we used for liver using anti-Cytochrome b561 D1 antibody A16342. Please let me know if you require anything else.
R. Yang
Verified customer
Asked: 2019-06-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-04
Question
Do you have a BSA free version of anti-Cytochrome b561 D1 antibody A16342 available?
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cytochrome b561 D1 antibody A16342 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-07
Question
Will anti-Cytochrome b561 D1 antibody A16342 work for WB with liver?
C. Lewis
Verified customer
Asked: 2015-08-11
Answer
According to the expression profile of liver, CYB561D1 is highly expressed in liver. So, it is likely that anti-Cytochrome b561 D1 antibody A16342 will work for WB with liver.
Boster Scientific Support
Answered: 2015-08-11
Question
Would A16342 anti-Cytochrome b561 D1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Mitchell
Verified customer
Asked: 2013-02-01
Answer
You can see on the product datasheet, A16342 anti-Cytochrome b561 D1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-02-01
