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Product Info Summary
| SKU: | A05107-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Cyt 19/As3mt Antibody Picoband®
SKU/Catalog Number
A05107-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cyt 19/As3mt Antibody Picoband® catalog # A05107-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyt 19/As3mt Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05107-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Cyt 19/As3mt recombinant protein (Position: M1-D346).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05107-2 is reactive to As3mt in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated molecular weight
75991 MW
Background of As3mt
AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism. This gene is mapped to chromosome 10q24.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05107-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: mouse liver tissue, mouse heart tissue, mouse Neuro-2a whole cell
FCM: mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cyt 19/As3mt using anti-Cyt 19/As3mt antibody (A05107-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse heart tissue lysates,
Lane 3: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyt 19/As3mt antigen affinity purified polyclonal antibody (Catalog # A05107-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyt 19/As3mt at approximately 44KD. The expected band size for Cyt 19/As3mt is at 42KD.
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Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-Cyt 19/As3mt antibody (A05107-2).
Overlay histogram showing A431 cells stained with A05107-2 (Blue line). To facilitate intracellular staining, tissues were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyt 19/As3mt Antibody (A05107-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For As3mt (Source: Uniprot.org, NCBI)
Gene Name
As3mt
Full Name
Arsenite methyltransferase
Weight
75991 MW
Superfamily
methyltransferase superfamily
Alternative Names
Sentrin-specific protease 6; SUMO-1-specific protease 1; Sentrin/SUMO-specific protease SENP6; SENP6; KIAA0797; SSP1; SUSP1; FKSG6 As3mt|2310045H08Rik, Cyt19|arsenite methyltransferase|arsenite methyltransferase|S-adenosyl-L-methionine:arsenic(III) methyltransferase|S-adenosylmethionine:arsenic (III) methyltransferase|arsenic (+3 oxidation state) methyltransferase|methylarsonite methyltransferase|methyltransferase Cyt19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on As3mt, check out the As3mt Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for As3mt: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyt 19/As3mt Antibody Picoband® (A05107-2)
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