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Product Info Summary
| SKU: | PA1888 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Krueppel-like factor 3 KLF3 Antibody Picoband®
SKU/Catalog Number
PA1888
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Krueppel-like factor 3 KLF3 Antibody catalog # PA1888. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Krueppel-like factor 3 KLF3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1888)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human KLF3, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1888 is reactive to KLF3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
18 kDa
Calculated molecular weight
38829 MW
Background of Klf3
KLF3 (Krueppel-like factor 3) is a protein that in humans is encoded by the KLF3 gene. It is also known as BKLF (Basic kruppel-like factor). KLF3 is a member of the Krüppel-like factors family of transcription factors. KLF3 primarily represses transcription through the transcriptional corepressor C-terminal binding protein (CtBP). Suske et al. (2005) stated that the human KLF3 gene maps to chromosome 4p14, and the mouse Klf3 gene maps to chromosome 5C3.3. Suske et al. (2005) stated that the human and mouse KLF3 proteins contain 345 and 344 amino acids, respectively. Both have a characteristic zinc finger domain, but lack the N-terminal buttonhead box found in related SP transcription factors. The repression mediated through KLF3 is also partially dependent on the sumoylation of its K10 and K197. Within KLF3's C-terminal, which is highly conserved throughout the KLF family, resides three C2H2 zinc fingers that preferentially bind the site NRG GNG NGR.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1888 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Testis Tissue, HELA Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-KLF3 antibody, PA1888, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: HELA Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For KLF3 (Source: Uniprot.org, NCBI)
Gene Name
KLF3
Full Name
Krueppel-like factor 3
Weight
38829 MW
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Krueppel-like factor 3;Basic krueppel-like factor;CACCC-box-binding protein BKLF;TEF-2;KLF3;BKLF; Klf3|9930027G08Rik, AL024007, BKL, Bklf, Tef-2|Kruppel-like factor 3 (basic)|Krueppel-like factor 3|CACCC-box-binding protein BKLF|basic krueppel-like factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLF3, check out the KLF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Krueppel-like factor 3 KLF3 Antibody Picoband® (PA1888)
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No publications found for PA1888
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Customer Q&As
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6 Customer Q&As for Anti-Krueppel-like factor 3 KLF3 Antibody Picoband®
Question
Does anti-KLF3 antibody PA1888 work on goat WB with cervix carcinoma?
R. Li
Verified customer
Asked: 2020-04-28
Answer
Our lab technicians have not validated anti-KLF3 antibody PA1888 on goat. You can run a BLAST between goat and the immunogen sequence of anti-KLF3 antibody PA1888 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-28
Question
Do you have a BSA free version of anti-KLF3 antibody PA1888 available?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-KLF3 antibody PA1888 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-03
Question
Can you help my question with product PA1888, anti-KLF3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1888 anti-KLF3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-KLF3 antibody PA1888. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-10
Question
We are currently using anti-KLF3 antibody PA1888 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
L. Williams
Verified customer
Asked: 2019-01-23
Answer
The anti-KLF3 antibody (PA1888) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-23
Question
Is a blocking peptide available for product anti-KLF3 antibody (PA1888)?
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
We do provide the blocking peptide for product anti-KLF3 antibody (PA1888). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-07
