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Product Info Summary
| SKU: | PB9494 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cystathionase/CTH Antibody Picoband®
View all Cystathionase Antibodies
SKU/Catalog Number
PB9494
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cystathionase/CTH Antibody Picoband® catalog # PB9494. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cystathionase/CTH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9494)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cystathionase recombinant protein (Position: D181-H398). Human Cystathionase shares 85.8% amino acid (aa) sequence identity with both mouse and rat Cystathionase.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9494 is reactive to CTH in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
44508 MW
Background of Cystathionase
Cystathionine gamma-lyase (or cystathionase) is an enzyme which breaks down cystathionine into cysteine and α-ketobutyrate. The International Radiation Hybrid Mapping Consortium mapped the CTH gene to chromosome 1. The CTH gene had earlier been assigned to chromosome 16 by study of somatic cell hybrids. It is demonstrated that hydrogen sulfide (H2S) is physiologically generated by CTH.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9494 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: K562 whole cell, HepG2 whole cell,, RH35 whole cell, rat liver tissue, rat kidney tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cystathionase using anti-Cystathionase antibody (PB9494).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: K562 whole cell lysates,
lane 2: HepG2 whole cell lysates,
lane 3: RH35 whole cell lysates,
lane 4: rat liver tissue lysates,
lane 5: rat kidney tissue lysates,
lane 6: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystathionase antigen affinity purified polyclonal antibody (Catalog # PB9494) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystathionase at approximately 45KD. The expected band size for Cystathionase is at 45KD.
Protein Target Info & Infographic
Gene/Protein Information For CTH (Source: Uniprot.org, NCBI)
Gene Name
CTH
Full Name
Cystathionine gamma-lyase
Weight
44508 MW
Superfamily
trans-sulfuration enzymes family
Alternative Names
Cystathionine gamma-lyase;4.4.1.1;Cysteine-protein sulfhydrase;Gamma-cystathionase;CTH; CTH cystathionine gamma-lyase cystathionine gamma-lyase|cystathionase (cystathionine gamma-lyase)|cysteine desulfhydrase|cysteine-protein sulfhydrase|gamma-cystathionase|homoserine deaminase|homoserine dehydratase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTH, check out the CTH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cystathionase/CTH Antibody Picoband® (PB9494)
Hello CJ!
PB9494 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Nie L,Liu M,Chen J,Wu Q,Li Y,Yi J,Zheng X,Zhang J,Chu C,Yang J.Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway.Mol Med Rep.2021 Apr;23(4):299.doi:10.3892/mmr.2021.11938.Epub 2021 Mar 2.PMID:336498
Species: Rat
PB9494 usage in article: APP:WB, SAMPLE:VENTRICULAR TISSUE, DILUTION:1:500
High C-X-C motif chemokine 5 expression is associated with malignant phenotypes of prostate cancer cells via autocrine and paracrine pathways
Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats
Endogenous carbon monoxide downregulates hepatic cystathionine-?-lyase in rats with liver cirrhosis
Caffeic Acid Phenethyl Ester inhibit Hepatic Fibrosis by Nitric Oxide Synthase and Cystathionine Gamma-Lyase in Rats
Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway
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Customer Q&As
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1 Customer Q&As for Anti-Cystathionase/CTH Antibody Picoband®
Question
We are currently using anti-Cystathionase/CTH antibody PB9494 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The anti-Cystathionase/CTH antibody (PB9494) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-03
