Product Info Summary
SKU: | M01601 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | WB |
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Product info
Product Name
Anti-CYP7A1 Monoclonal Antibody
SKU/Catalog Number
M01601
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CYP7A1 Monoclonal Antibody catalog # M01601. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYP7A1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01601)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
4D2
Isotype
IgG
Immunogen
Purified recombinant human CYP7A1 (C-terminal) protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01601 is reactive to CYP7A1 in Human
Applications
M01601 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
57661 MW
Background of CYP7A1
Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act as a cancer suppressor and inhibits malignant cell transformation. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Bin1 has also been implicated in Alzheimer disease and cardiac disease. Defects in Bin1 are the cause of centronuclear myopathy autosomal recessive; also known as autosomal recessive myotubular myopathy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis using CYP7A1 monoclonal antibody against Jurkat, K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CYP7A1 (Source: Uniprot.org, NCBI)
Gene Name
CYP7A1
Full Name
Cytochrome P450 7A1
Weight
57661 MW
Superfamily
cytochrome P450 family
Alternative Names
Cholesterol 7-alpha-hydroxylase; cholesterol 7-alpha-monooxygenase; CP7A; CYP7; CYP7A1; CYP7MGC126826; CYPVII; Cytochrome P450 7A1; cytochrome P450, family 7, subfamily A, polypeptide 1; cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase); cytochrome P450, subfamily VIIA polypeptide 1; EC 1.14.13; EC 1.14.13.17; MGC138389; polypeptide 1 CYP7A1 CP7A, CYP7, CYPVII cytochrome P450 family 7 subfamily A member 1 cytochrome P450 7A1|24-hydroxycholesterol 7-alpha-hydroxylase|cholesterol 7-alpha-hydroxylase|cholesterol 7-alpha-monooxygenase|cholesterol 7alpha-hydroxylase|cytochrome P450, family 7, subfamily A, polypeptide 1|cytochrome P450, subfamily VIIA polypeptide 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP7A1, check out the CYP7A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP7A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYP7A1 Monoclonal Antibody (M01601)
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Customer Q&As
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1 Customer Q&As for Anti-CYP7A1 Monoclonal Antibody
Question
What buffer is in M01601? Does it contain BSA?
Verified customer
Asked: 2021-02-08
Answer
For the Anti-CYP7A1 Monoclonal Antibody, purified mouse monoclonal in a buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 2% BSA,50% glycerol.
Boster Scientific Support
Answered: 2021-02-10