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Product Info Summary
| SKU: | A01737-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CYP4F2 Antibody Picoband®
SKU/Catalog Number
A01737-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CYP4F2 Antibody Picoband® catalog # A01737-2. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CYP4F2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01737-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CYP4F2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01737-2 is reactive to CYP4F2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated molecular weight
59.853kDa
Background of CYP4F2
Cytochrome P450 4F2 is a protein that in humans is encoded by the CYP4F2 gene. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F11, is approximately 16 kb away.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01737-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CYP4F2 using anti-CYP4F2 antibody (A01737-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP4F2 antigen affinity purified polyclonal antibody (A01737-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP4F2 at approximately 50 kDa. The expected band size for CYP4F2 is at 60 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CYP4F2 (Source: Uniprot.org, NCBI)
Gene Name
CYP4F2
Full Name
Cytochrome P450 4F2
Weight
59.853kDa
Superfamily
cytochrome P450 family
Alternative Names
CPF2; CYPIVF2; Cytochrome P450 4F2; cytochrome P450, family 4, subfamily F, polypeptide 2; cytochrome P450, subfamily IVF, polypeptide 2; Cytochrome P450-LTB-omega; EC 1.14.13.30; leukotriene B4 omega-hydroxylase; Leukotriene-B(4) 20-monooxygenase 1; leukotriene-B(4) omega-hydroxylase 1; leukotriene-B4 20-monooxygenase CYP4F2 CPF2 cytochrome P450 family 4 subfamily F member 2 cytochrome P450 4F2|20-HETE synthase|20-hydroxyeicosatetraenoic acid synthase|CYPIVF2|arachidonic acid omega-hydroxylase|cytochrome P450, family 4, subfamily F, polypeptide 2|cytochrome P450, subfamily IVF, polypeptide 2|cytochrome P450-LTB-omega|docosahexaenoic acid omega-hydroxylase|leukotriene-B(4) 20-monooxygenase 1|leukotriene-B(4) omega-hydroxylase 1|phylloquinone omega-hydroxylase CYP4F2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP4F2, check out the CYP4F2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP4F2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYP4F2 Antibody Picoband® (A01737-2)
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