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Product Info Summary
| SKU: | PA1699 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CYP11B1 Antibody Picoband®
SKU/Catalog Number
PA1699
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CYP11B1 Antibody catalog # PA1699. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYP11B1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1699)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CYP11B1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1699 is reactive to CYP11B1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
58 kDa
Calculated molecular weight
57573 MW
Background of CYP11B1
CYP11B1 (Cytochrome p450, family 11, subfamily B, polypeptide 1), also called Steroid 11-beta-hydroxylase or P450C11, is a steroid hydroxylase found in the zona fasciculata. The CYP11B1 gene functions primarily in mitochondria in the zona fasciculata of the adrenal cortex to convert 11-deoxycortisol to cortisol and 11-deoxycorticosterone to corticosterone. CYP11B1 is a member of the cytochrome P450 superfamily of enzymes. It is mapped on 8q24.3. The CYP11B1 gene contains 9 exons and spans 6.5 kb. Using RT-PCR, Kayes-Wandover and White detected CYP11B1 mRNA in human cardiac tissue samples from left and right atria, aorta, apex, intraventricular septum, and atrioventricular node, as well as whole adult and fetal heart. Ventricles did not express CYP11B1. In patients with 11-beta-hydroxylase deficiency leading to hypertension and congenital adrenal hyperplasia, Pascoe et al. identified mutations in the CYP11B1 gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1699 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, U87 Whole Cell, MM231 Whole Cell, PANC Whole Cell, MM453 Whole Cell, HELA Whole Cell, SMMC Whole Cell
Validation Images & Assay Conditions
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Anti-CYP11B1 antibody, PA1699, Western blotting
All lanes: Anti CYP11B1 (PA1699) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: MM453 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
Protein Target Info & Infographic
Gene/Protein Information For CYP11B1 (Source: Uniprot.org, NCBI)
Gene Name
CYP11B1
Full Name
Cytochrome P450 11B1, mitochondrial
Weight
57573 MW
Superfamily
cytochrome P450 family
Alternative Names
Cytochrome P450 11B1, mitochondrial;CYPXIB1;Cytochrome P-450c11;Cytochrome P450C11;Steroid 11-beta-hydroxylase;1.14.15.4;CYP11B1;S11BH; CYP11B1 CPN1, CYP11B, FHI, P450C11 cytochrome P450 family 11 subfamily B member 1 cytochrome P450 11B1, mitochondrial|cytochrome P-450c11|cytochrome P450, family 11, subfamily B, polypeptide 1|cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 1|cytochrome P450C11|cytochrome p450 XIB1|steroid 11-beta-hydroxylase, CYP11B1|steroid 11-beta-monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP11B1, check out the CYP11B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP11B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYP11B1 Antibody Picoband® (PA1699)
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No publications found for PA1699
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Customer Q&As
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1 Customer Q&As for Anti-CYP11B1 Antibody Picoband®
Question
We are currently using anti-CYP11B1 antibody PA1699 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
J. Baker
Verified customer
Asked: 2014-10-27
Answer
The anti-CYP11B1 antibody (PA1699) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-27
