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Product Info Summary
| SKU: | M00149-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00149-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody catalog # M00149-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00149-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DAD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclin D1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00149-1 is reactive to CCND1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
34 kDa
Calculated molecular weight
33729 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00149-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:100
IP 1:50
Positive Control
WB: human MCF-7 whole cell, human LNCAP whole cell, human HepG2 whole cell, human A431 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
IHC: human bladder tissue, Human pituitary tumor tissue, Human squamous cell carcinoma tissue, Human ovarian cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cyclin D1 using anti-Cyclin D1 antibody (M00149-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human LNCAP whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D1 antigen affinity purified monoclonal antibody (Catalog # M00149-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D1 at approximately 34 kDa. The expected band size for Cyclin D1 is at 34 kDa.
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Immunohistochemical analysis of paraffin-embedded human bladder, using Cyclin D1 Antibody.
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma , using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human ovarian cancer, using the Antibody at 1:500 dilution.
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Immunofluorescent analysis of MCF-7 cells, using Cyclin D1 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CCND1 (Source: Uniprot.org, NCBI)
Gene Name
CCND1
Full Name
G1/S-specific cyclin-D1
Weight
33729 MW
Superfamily
cyclin family
Alternative Names
G1/S-specific cyclin-D1;B-cell lymphoma 1 protein;BCL-1;BCL-1 oncogene;PRAD1 oncogene;CCND1;BCL1, PRAD1; CCND1 BCL1, D11S287E, PRAD1, U21B31 cyclin D1 G1/S-specific cyclin-D1|B-cell CLL/lymphoma 1|B-cell lymphoma 1 protein|BCL-1 oncogene|PRAD1 oncogene
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCND1, check out the CCND1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCND1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody (M00149-1)
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Customer Q&As
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1 Customer Q&As for Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody
Question
Could you ask Abways for the immunogen sequence of M00149-1 (CY5404) Cyclin D1?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The immunogen sequence of M00149-1 is VDLACTPTDVRDVDI.
Boster Scientific Support
Answered: 2020-04-30
