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Product Info Summary
| SKU: | PA1245-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cyclin D1/CCND1 Antibody Picoband®
SKU/Catalog Number
PA1245-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cyclin D1/CCND1 Antibody catalog # PA1245-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin D1/CCND1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1245-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cyclin D1, identical to the related rat sequence, and different from the related human sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1245-1 is reactive to Ccnd1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
54 kDa
Calculated molecular weight
33429 MW
Background of CCND1
Cyclin D1, also known as CCND1, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The cyclin D1 gene is overexpressed in human breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al. (2003) found that prolactin (PRL; 176760) induced IGF2 (147470) mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. And they also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and cyclin D1 are components of a developmental pathway in mammary gland.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1245-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Testis Tissue, Rat Ovary Tissue, Rat Brain Tissue, HELA Cell, MM231 Cell, SW620 Cell
Validation Images & Assay Conditions
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Anti-Cyclin D1 antibody, PA1245-1, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Ovary Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: MM231 Cell Lysate
Lane 6: SW620 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For Ccnd1 (Source: Uniprot.org, NCBI)
Gene Name
Ccnd1
Full Name
G1/S-specific cyclin-D1
Weight
33429 MW
Superfamily
cyclin family
Alternative Names
G1/S-specific cyclin-D1;Ccnd1;Cyl-1; CCND1 BCL1, D11S287E, PRAD1, U21B31 cyclin D1 G1/S-specific cyclin-D1|B-cell CLL/lymphoma 1|B-cell lymphoma 1 protein|BCL-1 oncogene|PRAD1 oncogene
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ccnd1, check out the Ccnd1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ccnd1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin D1/CCND1 Antibody Picoband® (PA1245-1)
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Customer Q&As
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6 Customer Q&As for Anti-Cyclin D1/CCND1 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Cyclin D1/CCND1 antibody PA1245-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-24
Question
I see that the anti-Cyclin D1/CCND1 antibody PA1245-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-19
Question
I am interested in to test anti-Cyclin D1/CCND1 antibody PA1245-1 on rat liver for research purposes, then I may be interested in using anti-Cyclin D1/CCND1 antibody PA1245-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The products we sell, including anti-Cyclin D1/CCND1 antibody PA1245-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-26
Question
Would anti-Cyclin D1/CCND1 antibody PA1245-1 work on bovine WB with placenta?
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
Our lab technicians have not tested anti-Cyclin D1/CCND1 antibody PA1245-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Cyclin D1/CCND1 antibody PA1245-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-07
Question
Is this PA1245-1 anti-Cyclin D1/CCND1 antibody reactive to the isotypes of CCND1?
C. Kulkarni
Verified customer
Asked: 2017-11-10
Answer
The immunogen of PA1245-1 anti-Cyclin D1/CCND1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cyclin D1(219-238aa SPNNFLSCYRTTHFLSRVIK), identical to the related rat sequence, and different from the related human sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-10
Question
I was wanting to use your anti-Cyclin D1/CCND1 antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
O. Moore
Verified customer
Asked: 2015-06-25
Answer
You can see on the product datasheet, PA1245-1 anti-Cyclin D1/CCND1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-06-25
