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Product Info Summary
| SKU: | A04464 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-CYB5 Antibody
View all Cytochrome b5 type A Antibodies
SKU/Catalog Number
A04464
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CYB5 Antibody (Catalog # A04464). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYB5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04464)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CYB5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04464 is reactive to CYB5A in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
15330 MW
Background of Cytochrome b5 type A
Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Yoo M., Biochem. Biophys. Res. Commun. 156:576-580(1988).
Li X.R., Biochem. Biophys. Res. Commun. 209:894-900(1995).
Giordano S.J., Biochem. Biophys. Res. Commun. 178:38-44(1991).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04464 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from A549 cells, using CYB5 antibody A04464.
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Immunofluorescence analysis of NIH/3T3 cells, using CYB5 antibody A04464.
Protein Target Info & Infographic
Gene/Protein Information For CYB5A (Source: Uniprot.org, NCBI)
Gene Name
CYB5A
Full Name
Cytochrome b5
Weight
15330 MW
Superfamily
cytochrome b5 family
Alternative Names
Cytochrome b5;Microsomal cytochrome b5 type A;MCB5;CYB5A;CYB5; CYB5A CYB5, MCB5, METAG cytochrome b5 type A cytochrome b5|cytochrome b5 type A (microsomal)|epididymis secretory sperm binding protein|type 1 cyt-b5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYB5A, check out the CYB5A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYB5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYB5 Antibody (A04464)
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No publications found for A04464
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-CYB5 Antibody
Question
Will anti-CYB5 antibody A04464 work on monkey WB with liver?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Our lab technicians have not tested anti-CYB5 antibody A04464 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-CYB5 antibody A04464 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-20
Question
I have a question about product A04464, anti-CYB5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04464 anti-CYB5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-30
Question
Is a blocking peptide available for product anti-CYB5 antibody (A04464)?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
We do provide the blocking peptide for product anti-CYB5 antibody (A04464). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-18
Question
Do you have a BSA free version of anti-CYB5 antibody A04464 available?
B. Singh
Verified customer
Asked: 2019-09-02
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CYB5 antibody A04464 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-02
Question
We are currently using anti-CYB5 antibody A04464 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The anti-CYB5 antibody (A04464) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-15
Question
Is this A04464 anti-CYB5 antibody reactive to the isotypes of CYB5A?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
The immunogen of A04464 anti-CYB5 antibody is Synthesized peptide derived from internal of human CYB5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-04
Question
I was wanting to use your anti-CYB5 antibody for IF for human uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human uterus identification?
Verified Customer
Verified customer
Asked: 2019-01-03
Answer
You can see on the product datasheet, A04464 anti-CYB5 antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-03
Question
I have attached the WB image, lot number and protocol we used for uterus using anti-CYB5 antibody A04464. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-19
Question
My lab would like to test anti-CYB5 antibody A04464 on human uterus for research purposes, then I may be interested in using anti-CYB5 antibody A04464 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Jha
Verified customer
Asked: 2018-07-31
Answer
The products we sell, including anti-CYB5 antibody A04464, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-31
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for uterus using anti-CYB5 antibody A04464. Let me know if you need anything else.
D. Jha
Verified customer
Asked: 2018-04-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-03
Question
Will anti-CYB5 antibody A04464 work for IF with uterus?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
According to the expression profile of uterus, CYB5A is highly expressed in uterus. So, it is likely that anti-CYB5 antibody A04464 will work for IF with uterus.
Boster Scientific Support
Answered: 2018-03-22
Question
Will A04464 anti-CYB5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
It shows on the product datasheet, A04464 anti-CYB5 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-27
Question
I see that the anti-CYB5 antibody A04464 works with IF, what is the protocol used to produce the result images on the product page?
M. Krishna
Verified customer
Asked: 2016-12-28
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-12-28
