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Product Info Summary
| SKU: | A00663-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-Cxcr5 Antibody
SKU/Catalog Number
A00663-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cxcr5 Antibody catalog # A00663-1. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Cxcr5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00663-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Cxcr5 recombinant protein (Position: M1-F374).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00663-1 is reactive to Cxcr5 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
41.955kDa
Background of CXCR5
CXCR5(Chemokine CXC Motif Receptor 5), also known as BLR1, is a G protein-coupled seven transmembrane receptor for chemokine CXCL13(also known as BLC) and belongs to the CXC chemokine receptor family. In humans, the CXC-R5 protein is encoded by the CXCR5 gene. The gene plays an essential role in B cell migration. Reif et al.(2002) concluded that their findings defined the mechanism of B-cell relocalization in response to antigen, and established that cell position in vivo can be determined by the balance of responsiveness to chemoattractants made in separate but adjacent zones. Chan et al.(2003) investigated the expression of chemokines and chemokine receptors in eyes with primary intraocular B-cell lymphoma(PIOL).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00663-1 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
IHC: mouse spleen tissue, rat lymph nodes tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of TFEB using anti-TFEB antibody (A00663-1).
TFEB was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFEB Antibody (A00663-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 2. IHC analysis of TFEB using anti-TFEB antibody (A00663-1).
TFEB was detected in a paraffin-embedded section of rat lymph nodes tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFEB Antibody (A00663-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Cxcr5 (Source: Uniprot.org, NCBI)
Gene Name
Cxcr5
Full Name
C-X-C chemokine receptor type 5
Weight
41.955kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
NKG2-D type II integral membrane protein; Killer cell lectin-like receptor subfamily K member 1; NK cell receptor D; NKG2-D-activating NK receptor; CD314; KLRK1; D12S2489E; NKG2D CXCR5 BLR1, CD185, MDR15 C-X-C motif chemokine receptor 5 C-X-C chemokine receptor type 5|Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5)|Burkitt lymphoma receptor 1, GTP-binding protein|CXC-R5|CXCR-5|MDR-15|chemokine (C-X-C motif) receptor 5|monocyte-derived receptor 15
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cxcr5, check out the Cxcr5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cxcr5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cxcr5 Antibody (A00663-1)
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