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Product Info Summary
| SKU: | PB9694 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MIG/CXCL9 Antibody Picoband®
SKU/Catalog Number
PB9694
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MIG/CXCL9 Antibody Picoband® catalog # PB9694. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MIG/CXCL9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9694)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CXCL9 recombinant protein (Position: T23-T125). Human CXCL9 shares 68.2% amino acid (aa) sequence identity with mouse CXCL9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9694 is reactive to CXCL9 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
14019 MW
Background of CXCL9/MIG
MIG, also known as CXCL9, is a T-cell chemoattractant inducible by gamma interferon that is a member of the CXC chemokine family of cytokines. This gene is mapped to 4q21. It is noted that, although the best-described activities of the chemokines are as chemotactic factors, chemokines also have an effect on T-cell activation, angiogenesis, and HIV infection. While most CXC chemokines are chemotactic for neutrophils, MIG and INP10 are unusual and similar in being CXC chemokines that are chemotactic for lymphocytes and inactive in neutrophils.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9694 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: Human Placenta Tissue, A431 Whole Cell, HELA Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CXCL9 using anti-CXCL9 antibody (PB9694).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Human Placenta Tissue Lysate at 50ug,
Lane 2: A431 Whole Cell Lysate at 40ug,
Lane 3: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL9 antigen affinity purified polyclonal antibody (Catalog # PB9694) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL9 at approximately 19 kDa. The expected band size for CXCL9 is at 14 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CXCL9 (Source: Uniprot.org, NCBI)
Gene Name
CXCL9
Full Name
C-X-C motif chemokine 9
Weight
14019 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
C-X-C motif chemokine 9;Gamma-interferon-induced monokine;Monokine induced by interferon-gamma;HuMIG;MIG;Small-inducible cytokine B9;CXCL9;CMK, MIG, SCYB9; CXCL9 CMK, Humig, MIG, SCYB9, crg-10 C-X-C motif chemokine ligand 9 C-X-C motif chemokine 9|chemokine (C-X-C motif) ligand 9|gamma-interferon-induced monokine|monokine induced by gamma interferon|monokine induced by interferon-gamma|small-inducible cytokine B9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCL9, check out the CXCL9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCL9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MIG/CXCL9 Antibody Picoband® (PB9694)
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Customer Q&As
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1 Customer Q&As for Anti-MIG/CXCL9 Antibody Picoband®
Question
We are currently using anti-MIG/CXCL9 antibody PB9694 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
The anti-MIG/CXCL9 antibody (PB9694) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-08
