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- Table of Contents
Facts about Cullin-2.
May serve as a rigid scaffold from the complex and might contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of this complex is determined by the neddylation of the cullin subunit and is inhibited by the association of this deneddylated cullin subunit with TIP120A/CAND1.
Human | |
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Gene Name: | CUL2 |
Uniprot: | Q13617 |
Entrez: | 8453 |
Belongs to: |
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cullin family |
CUL2; CUL-2; Cullin 2; cullin-2; MGC131970
Mass (kDA):
86.983 kDA
Human | |
---|---|
Location: | 10p11.21 |
Sequence: | 10; NC_000010.11 (35008551..35126899, complement) |
Boster Bio's research primary antibodies and secondary antibodies are a great way to optimize your ELISA results. Then, you can optimize your experiments using the Boster Bio optimization guides and tips. There are many choices, and the guide will answer your questions about the various flow procedures and optimization tips. You can read on to find out more. Below are the top three uses of CUL2 Marker.
Boster Bio's primary, secondary and hybrid antibodies are used for biomarker identification in many different areas. They can detect biomarkers related to neuroscience, cancer, and development biology. The research antibodies kits are designed to have picogram-level specificity. Boster Bio immunological products can be ordered through tebu.bio. In this article, we'll discuss how to use Boster Bio's primary and secondary antibodies in your research.
To evaluate a primary antibody's ability to recognize the antigen expected epitope, it must be able. This is called specificity and can be verified by immunoblotting on fixed cells. Although it's simple and cheap, labeling proteins causes some of their secondary or tertiary structure to be lost. Some antibodies are selective for proteins that have been denatured, while others only bind to native proteins.
The primary antibody's specificity is verified by performing a number of different experiments. Primary antibodies can be used in multiple experiments that measure their binding to the antigen. One of the best methods to test for antigen binding is immunoblotting tissue with a fluorescent protein like GFP. A second technique, immunocytochemistry that uses a secondary antigen to recognize the same antigen can be used to determine its specificity.
Boster Bio's PICOKINE (tm) ELISA kits for the detection of the CUL2 marker were developed through 20 years of research and development. Picokine technology enhances the binding efficiency and decreases non-specific binding. This allows them to achieve high specificity and picogram-level sensitiveness. They are highly sensitive and have enough reagents to run 96 tests.
For ELISA to work, plates must be coated in a way that allows for a reproducible and sensitive detection range. Microplates should have a protein binding capacity of at most 400 ng/cm2 with a CV value below one. Nonspecific binding can be avoided by having the right coating conditions. In contrast, a low CV value ensures reproducible results. The type of signal is detected determines the color of the plates. Colorimetric signals will be detected by using flat bottom plates made of clear polystyrene, while fluorescent signals will require opaque microplates made of black or white.
The PICOBAND(tm), sensitivity ELISA platform was designed for applications that require high sensitivity in quantification of CUL2 markers. The ELISA kits can be validated on a variety of samples and a detailed validation process is available upon request. Supervision, a secondary antibody made from polymers, is included in the kits. It allows for faster IHC. PicoBAND (tm) and Supervision were developed from insights into immunogen engineering. Technical support is provided by Sanbio, a BeNeLux distributor.
For more information on the PICOBAND(tm) ELIISA kits for the CUL2 marker, contact Sanbio at [email protected]. The company also offers custom-services and BeNeLux shipping. They are happy to answer any questions. These kits deliver high-quality results in under an hour. You can expect fast and reliable delivery with the PICOBAND(tm) ELISA kits.
PMID: 9122164 by Pause A., et al. The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins.
PMID: 10092517 by Wada H., et al. Identification of NEDD8-conjugation site in human cullin-2.