Product Info Summary
SKU: | A03496 |
---|---|
Size: | 50 µg |
Reactive Species: | Bovine, Mouse, Rat, Sheep, Porcine |
Host: | Goat |
Application: | IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CRYAB/Alpha B Crystallin Antibody
View all AlphaB Crystallin/CRYAB Antibodies
SKU/Catalog Number
A03496
Size
50 µg
Form
Liquid
Description
Boster Bio Anti-CRYAB/Alpha B Crystallin Antibody catalog # A03496. Tested in WB, IP applications. This antibody reacts with Mouse, Rat, Bovine, Porcine, Sheep.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CRYAB/Alpha B Crystallin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03496)
Host
Goat
Contents
In sodium phosphate buffer, pH 7.0, containing 0.1% sodium azide, 3% BSA, and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Standard Protein
Synthetic peptide corresponding to to aa 54-64 of of human αB-crystallin phosphorylated at Ser59.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03496 is reactive to CRYAB in Bovine, Mouse, Rat, Sheep, Porcine
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
20037 MW
Background of AlphaB Crystallin/CRYAB
The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.
Kelian Chen, et,al. (2003) J. Cell Sci ; 117: 4527 - 4536.
Vera Strasser, et,al. (2004) Mol. Cell. Biol ; 24: 1378 - 1386.
Izhar Ben-Shlomo, et,al. (2003) Sci. STKE ; 2003: 9.
H. M. Kim, et,al. (2002) PNAS ; 99: 4020.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03496 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, ECL, 1:1, 000
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
![A03496_1 WB anti cryab alpha b crystallin antibody A03496_1 WB anti cryab alpha b crystallin antibody](https://www.bosterbio.com/media/catalog/product/antibody/A03496_1-WB-anti-cryab-alpha-b-crystallin-antibody.jpg)
Click image to see more details
Western blot analysis of PSer 59 Alpha B-Crystallin expression in MW marker (lane 1), native bovine α-Crystallin protein (lane 2), native bovine β-Crystallin protein (lane 3), mouse heart extract (lane 4), rat heart extract (lane 5) and ESK-4 whole cell lysates (lane 6). PSer 59 Alpha B-Crystallin was detected using rabbit anti-PSer 59 Alpha B-Crystallin polyclonal antibody (Catalog # A03496) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For CRYAB (Source: Uniprot.org, NCBI)
Gene Name
CRYAB
Full Name
Alpha-crystallin B chain
Weight
20037 MW
Superfamily
small heat shock protein (HSP20) family
Alternative Names
alpha B crystallin; alpha(B)-crystallin; AlphaB Crystallin; alpha-crystallin B chain; CRYA2; CRYA2alpha crystallin B chain; CRYAB; crystallin, alpha B; CTPP2; Heat shock protein beta-5; heat-shock 20 kD like-protein; HSPB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component CRYAB CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2 crystallin alpha B alpha-crystallin B chain|epididymis secretory protein Li 101|heat shock protein beta-5|heat-shock 20 kD like-protein|renal carcinoma antigen NY-REN-27|rosenthal fiber component
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRYAB, check out the CRYAB Infographic
![CRYAB infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRYAB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CRYAB/Alpha B Crystallin Antibody (A03496)
Hello CJ!
No publications found for A03496
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CRYAB/Alpha B Crystallin Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CRYAB/Alpha B Crystallin Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-CRYAB/Alpha B Crystallin Antibody
Question
Does anti-CRYAB/Alpha B Crystallin antibody A03496 work for WB with liver?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
According to the expression profile of liver, CRYAB is highly expressed in liver. So, it is likely that anti-CRYAB/Alpha B Crystallin antibody A03496 will work for WB with liver.
Boster Scientific Support
Answered: 2020-02-26
Question
I see that the anti-CRYAB/Alpha B Crystallin antibody A03496 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-27
Question
Will A03496 anti-CRYAB/Alpha B Crystallin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
You can see on the product datasheet, A03496 anti-CRYAB/Alpha B Crystallin antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-10
Question
My question regarding product A03496, anti-CRYAB/Alpha B Crystallin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03496 anti-CRYAB/Alpha B Crystallin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-10
Question
I was wanting to use your anti-CRYAB/Alpha B Crystallin antibody for WB for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
As indicated on the product datasheet, A03496 anti-CRYAB/Alpha B Crystallin antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-01
Question
See below the WB image, lot number and protocol we used for liver using anti-CRYAB/Alpha B Crystallin antibody A03496. Please let me know if you require anything else.
D. Li
Verified customer
Asked: 2016-01-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-01