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Product Info Summary
| SKU: | M30929 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Streptococcus pyogenes, Recombinant fragment |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody
SKU/Catalog Number
M30929
BM5120 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody catalog # M30929. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Recombinant fragment, Streptococcus pyogenes.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30929)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABCI-3
Isotype
Rabbit IgG
Immunogen
Recombinant fragment derived from Streptococcus pyogenes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M30929 is reactive to cas9 in Streptococcus pyogenes, Recombinant fragment
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
158441 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30929 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: 293T cell, 293T cell, 3T3 cell, PC12 cell
ICC/IF: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate.
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Immunofluorescent analysis of 293T cells transfected with CRISPR-SpCas9, using CRISPR-Cas9 SP Antibody .
Protein Target Info & Infographic
Gene/Protein Information For cas9 (Source: Uniprot.org, NCBI)
Gene Name
cas9
Full Name
Weight
158441 MW
Alternative Names
CRISPR-associated endonuclease Cas9/Csn1 ;3.1.-.- ;SpCas9 ;SpyCas9 ;cas9 ;csn1 ;SPy_1046;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on cas9, check out the cas9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for cas9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody (M30929)
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No publications found for M30929
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