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Product Info Summary
| SKU: | PA1852 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Coxsackie Adenovirus Receptor/CXADR Antibody Picoband®
SKU/Catalog Number
PA1852
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Coxsackie Adenovirus Receptor/CXADR Antibody catalog # PA1852. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Coxsackie Adenovirus Receptor/CXADR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1852)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Coxsackie Adenovirus Receptor, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1852 is reactive to CXADR in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
40030 MW
Background of CXADR
CXADR (Coxsackie virus and adenovirus receptor) is a protein that in humans is encoded by the CXADR gene, also known as CAR,CVB3-binding protein, Coxsackievirus B-adenovirus receptor. The CAR cDNA encodes a predicted 365-amino acid polypeptide that contains a single transmembrane domain and is a member of the immunoglobulin superfamily. By Northern blot analysis, they detected highest expression of 1.4-kb and 6-kb CXADR transcripts in pancreas, brain, heart, small intestine, testis, and prostate, lower expression in liver and lung, and no expression in kidney, placenta, peripheral blood leukocytes, thymus, and spleen. In comparison, mouse Cxadr showed highest expression in liver, and lower levels in kidney, heart, lung, and brain. The protein encoded by this gene is a type I membrane receptor for group B coxsackie viruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. CAR is strongly expressed in the developing central nervous system. It functions as a homophilic and also as a heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins , such as: fibronectin, agrin, laminin-1 and tenascin-R.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1852 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat liver tissue, rat heart tissue, mouse liver tissue, mouse HEPA1-6 whole cell, human A431 whole cell, human Hela whole cell, human HepG2 whole cell, human Caco-2 whole cell, human U-937whole cell(negative), human THP-1whole cell(negative)
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody (PA1852).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysate,
Lane 2: rat heart tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody (Catalog # PA1852) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.
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Figure 1. Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody (PA1852).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: human U-937whole cell lysates(negative),
Lane 6: human THP-1whole cell lysates(negative).
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody (Catalog # PA1852) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.
Protein Target Info & Infographic
Gene/Protein Information For CXADR (Source: Uniprot.org, NCBI)
Gene Name
CXADR
Full Name
Coxsackievirus and adenovirus receptor
Weight
40030 MW
Alternative Names
Coxsackievirus and adenovirus receptor;CAR;hCAR;CVB3-binding protein;Coxsackievirus B-adenovirus receptor;HCVADR;CXADR;CAR; CXADR CAR, CAR4/6, HCAR CXADR Ig-like cell adhesion molecule coxsackievirus and adenovirus receptor|46 kD coxsackievirus and adenovirus receptor (CAR) protein|CVB3-binding protein|HCVADR|coxsackie virus and adenovirus receptor|coxsackievirus B-adenovirus receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXADR, check out the CXADR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXADR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Coxsackie Adenovirus Receptor/CXADR Antibody Picoband® (PA1852)
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Customer Q&As
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1 Customer Q&As for Anti-Coxsackie Adenovirus Receptor/CXADR Antibody Picoband®
Question
We are currently using anti-Coxsackie Adenovirus Receptor/CXADR antibody PA1852 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
A. Johnson
Verified customer
Asked: 2017-11-06
Answer
The anti-Coxsackie Adenovirus Receptor/CXADR antibody (PA1852) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-06
