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Product Info Summary
| SKU: | M01026 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Collagen X COL10A1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01026
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Collagen X COL10A1 Rabbit Monoclonal Antibody catalog # M01026. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen X COL10A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01026)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOAH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Collagen X
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01026 is reactive to COL10A1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
66 kDa
Calculated molecular weight
66 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01026 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: mouse liver tissue, rat liver tissue, human fetal skin
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of COL10A1 using anti-COL10A1 antibody (M01026).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL10A1 antigen affinity purified monoclonal antibody (Catalog # M01026) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL10A1 at approximately 66 kDa. The expected band size for COL10A1 is at 66 kDa.
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Western blot analysis of Collagen X expression in human fetal skin lysate.
Protein Target Info & Infographic
Gene/Protein Information For COL10A1 (Source: Uniprot.org, NCBI)
Gene Name
COL10A1
Full Name
Collagen alpha-1(X) chain
Weight
66 kDa
Alternative Names
Collagen alpha-1 (X) chain;COL10A1; COL10A1 collagen type X alpha 1 chain collagen alpha-1(X) chain|Schmid metaphyseal chondrodysplasia|collagen X, alpha-1 polypeptide|collagen, type X, alpha 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL10A1, check out the COL10A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL10A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen X COL10A1 Rabbit Monoclonal Antibody (M01026)
Hello CJ!
M01026 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chaoyue Hong,Yi Tang,Xiu Hu,Xiwen Song,Yun Cai,Zhaohui Song,Feiwu Kang,Partial deficiency of HIF-1α in chondrocytes effected bone repair of mandibular condylar neck, Archives of Oral Biology, 2020, 105023, ISSN 0003-9969, https://doi.org/10.1016/j.archora
Species: Mouse
M01026 usage in article: APP:IF, SAMPLE:CHONDROCYTES, DILUTION:1:100
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Customer Q&As
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6 Customer Q&As for Anti-Collagen X COL10A1 Rabbit Monoclonal Antibody
Question
I have a question about product M01026, anti-Collagen X Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01026 anti-Collagen X Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-31
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tibia using anti-Collagen X Rabbit Monoclonal antibody M01026. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-25
Question
We need to test anti-Collagen X Rabbit Monoclonal antibody M01026 on mouse tibia for research purposes, then I may be interested in using anti-Collagen X Rabbit Monoclonal antibody M01026 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
The products we sell, including anti-Collagen X Rabbit Monoclonal antibody M01026, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-06
Question
Is there a BSA free version of anti-Collagen X Rabbit Monoclonal antibody M01026 available?
Verified Customer
Verified customer
Asked: 2019-02-07
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Collagen X Rabbit Monoclonal antibody M01026 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-07
Question
I was wanting to use your anti-Collagen X Rabbit Monoclonal antibody for WB for mouse tibia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse tibia identification?
Verified Customer
Verified customer
Asked: 2018-04-10
Answer
As indicated on the product datasheet, M01026 anti-Collagen X Rabbit Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse tibia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-10
Question
We are currently using anti-Collagen X Rabbit Monoclonal antibody M01026 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
The anti-Collagen X Rabbit Monoclonal antibody (M01026) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-20
