Click image to see more details
Product Info Summary
| SKU: | A02844 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Collagen VI alpha3 COL6A3 Antibody
View all Collagen VI alpha 3 Antibodies
SKU/Catalog Number
A02844
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Collagen VI alpha3 COL6A3 Antibody (Catalog# A02844). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen VI alpha3 COL6A3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02844)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Collagen VI alpha3. Immunogen sequence location: 2261-2310
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02844 is reactive to COL6A3 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
343669 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02844 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen VI alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.
Click image to see more details
Immunofluorescence analysis of HeLa cells, using Collagen VI alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For COL6A3 (Source: Uniprot.org, NCBI)
Gene Name
COL6A3
Full Name
Collagen alpha-3(VI) chain
Weight
343669 MW
Superfamily
type VI collagen family
Alternative Names
COL6A3;Collagen alpha-3(VI) chain COL6A3 BTHLM1, DYT27, UCMD1 collagen type VI alpha 3 chain collagen alpha-3(VI) chain|collagen VI, alpha-3 polypeptide|collagen, type VI, alpha 3|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL6A3, check out the COL6A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL6A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen VI alpha3 COL6A3 Antibody (A02844)
Hello CJ!
No publications found for A02844
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Collagen VI alpha3 COL6A3 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Collagen VI alpha3 COL6A3 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Collagen VI alpha3 COL6A3 Antibody
Question
Will anti-Collagen VI alpha3 antibody A02844 work on bovine IHC with uterine cervix?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
Our lab technicians have not validated anti-Collagen VI alpha3 antibody A02844 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Collagen VI alpha3 antibody A02844 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine uterine cervix in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-31
Question
We are currently using anti-Collagen VI alpha3 antibody A02844 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The anti-Collagen VI alpha3 antibody (A02844) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-29
Question
Would A02844 anti-Collagen VI alpha3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
You can see on the product datasheet, A02844 anti-Collagen VI alpha3 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-08
Question
Here is the WB image, lot number and protocol we used for plasma using anti-Collagen VI alpha3 antibody A02844. Please let me know if you require anything else.
W. Anderson
Verified customer
Asked: 2019-10-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-17
Question
Is this A02844 anti-Collagen VI alpha3 antibody reactive to the isotypes of COL6A3?
Verified Customer
Verified customer
Asked: 2018-08-07
Answer
The immunogen of A02844 anti-Collagen VI alpha3 antibody is A peptide derived from human Collagen VI alpha3. Immunogen sequence location: 2261-2310. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-07
Question
I have a question about product A02844, anti-Collagen VI alpha3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Carter
Verified customer
Asked: 2018-05-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02844 anti-Collagen VI alpha3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-24
Question
I see that the anti-Collagen VI alpha3 antibody A02844 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-18
