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Product Info Summary
| SKU: | A10525 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Collagen alpha-3(V) chain COL5A3 Antibody
View all Collagen V alpha 3 Antibodies
SKU/Catalog Number
A10525
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Collagen alpha-3 (V) chain COL5A3 Antibody catalog # A10525. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen alpha-3(V) chain COL5A3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10525)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Collagen V alpha3. AA range:221-270
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10525 is reactive to COL5A3 in Human
Reconstitution
Calculated molecular weight
172121 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10525 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of K562 cells using COL5A3 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using COL5A3 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For COL5A3 (Source: Uniprot.org, NCBI)
Gene Name
COL5A3
Full Name
Collagen alpha-3(V) chain
Weight
172121 MW
Superfamily
fibrillar collagen family
Alternative Names
Collagen alpha-3 (V) chain;COL5A3; COL5A3 collagen type V alpha 3 chain collagen alpha-3(V) chain|collagen, type V, alpha 3|pro-(alpha)3(V) collagen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL5A3, check out the COL5A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL5A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen alpha-3(V) chain COL5A3 Antibody (A10525)
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No publications found for A10525
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Customer Q&As
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4 Customer Q&As for Anti-Collagen alpha-3(V) chain COL5A3 Antibody
Question
We are currently using anti-COL5A3 antibody A10525 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The anti-COL5A3 antibody (A10525) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-10
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-COL5A3 antibody A10525. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-02
Question
Do you have a BSA free version of anti-COL5A3 antibody A10525 available?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-COL5A3 antibody A10525 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-31
Question
I see that the anti-COL5A3 antibody A10525 works with WB, what is the protocol used to produce the result images on the product page?
K. Moore
Verified customer
Asked: 2017-10-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-05
