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Product Info Summary
| SKU: | A03869 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Collagen alpha-2(V) chain COL5A2 Antibody
View all Collagen V alpha 2 Antibodies
SKU/Catalog Number
A03869
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Collagen alpha-2 (V) chain COL5A2 Antibody catalog # A03869. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen alpha-2(V) chain COL5A2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03869)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the N-terminal region of human COL5A2. AA range:1-50
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03869 is reactive to COL5A2 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
144910 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03869 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HepG2 cells using COL5A2 Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Pancreas, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For COL5A2 (Source: Uniprot.org, NCBI)
Gene Name
COL5A2
Full Name
Collagen alpha-2(V) chain
Weight
144910 MW
Superfamily
fibrillar collagen family
Alternative Names
Collagen alpha-2 (V) chain;COL5A2; COL5A2 EDSC, EDSCL2 collagen type V alpha 2 chain collagen alpha-2(V) chain|AB collagen|collagen, fetal membrane, A polypeptide|type V preprocollagen alpha 2 chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL5A2, check out the COL5A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL5A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen alpha-2(V) chain COL5A2 Antibody (A03869)
Hello CJ!
A03869 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Identifying miRNAs in multiple sclerosis gray matter lesions that correlate with atrophy measures
hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Collagen alpha-2(V) chain COL5A2 Antibody
Question
I am looking for to test anti-COL5A2 antibody A03869 on human bone for research purposes, then I may be interested in using anti-COL5A2 antibody A03869 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The products we sell, including anti-COL5A2 antibody A03869, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-29
Question
Is a blocking peptide available for product anti-COL5A2 antibody (A03869)?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
We do provide the blocking peptide for product anti-COL5A2 antibody (A03869). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-29
Question
We are currently using anti-COL5A2 antibody A03869 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
The anti-COL5A2 antibody (A03869) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-12
Question
I see that the anti-COL5A2 antibody A03869 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-02
Question
Can you help my question with product A03869, anti-COL5A2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Johnson
Verified customer
Asked: 2017-12-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03869 anti-COL5A2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-01
Question
Would anti-COL5A2 antibody A03869 work for WB with bone?
G. Bhatt
Verified customer
Asked: 2014-05-08
Answer
According to the expression profile of bone, COL5A2 is highly expressed in bone. So, it is likely that anti-COL5A2 antibody A03869 will work for WB with bone.
Boster Scientific Support
Answered: 2014-05-08
