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Product Info Summary
| SKU: | RP1107 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Cofilin 2/CFL2 Antibody Picoband®
SKU/Catalog Number
RP1107
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cofilin 2/CFL2 Antibody catalog # RP1107. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cofilin 2/CFL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1107)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin 2, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1107 is reactive to CFL2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
18,737 MW
Background of Cofilin 2
Cofilin 2 (muscle), also known as CFL2, is a protein which in humans is encoded by the CFL2 gene. It is mapped to 14q12. This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. And this protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1107 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Raji whole cell lysates, human HUVEC whole cell lysates, human HepG2 whole cell lysates, human Hela whole cell lysates, rat skeletal muscle tissue lysates, rat RH35 whole cell lysates, rat liver tissue lysates, mouse skeletal muscle tissue lysates, mouse liver tissue lysates
ICC/IF: U2OS cells
FCM: Raji cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cofilin 2 using anti-Cofilin 2 antibody (RP1107).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human HUVEC whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: rat liver tissue lysates,
Lane 8: mouse skeletal muscle tissue lysates,
Lane 9: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cofilin 2 antigen affinity purified polyclonal antibody (Catalog # RP1107) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cofilin 2 at approximately 19 kDa. The expected band size for Cofilin 2 is at 19 kDa.
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Figure 2. IF analysis of Cofilin 2 using anti-Cofilin 2 antibody (RP1107).
Cofilin 2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cofilin 2 Antibody (RP1107) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of Raji cells using anti-Cofilin 2 antibody (RP1107).
Overlay histogram showing Raji cells stained with RP1107 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cofilin 2 Antibody (RP1107, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CFL2 (Source: Uniprot.org, NCBI)
Gene Name
CFL2
Full Name
Cofilin-2
Weight
18,737 MW
Superfamily
actin-binding proteins ADF family
Alternative Names
Cofilin-2;Cofilin, muscle isoform;CFL2; CFL2 NEM7 cofilin 2 cofilin-2|cofilin 2 (muscle)|nemaline myopathy type 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CFL2, check out the CFL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CFL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cofilin 2/CFL2 Antibody Picoband® (RP1107)
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Customer Q&As
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3 Customer Q&As for Anti-Cofilin 2/CFL2 Antibody Picoband®
Question
Can you help my question with product RP1107, anti-Cofilin 2/CFL2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1107 anti-Cofilin 2/CFL2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-01
Question
We are currently using anti-Cofilin 2/CFL2 antibody RP1107 for rat tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
The anti-Cofilin 2/CFL2 antibody (RP1107) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-06
Question
Will anti-Cofilin 2/CFL2 antibody RP1107 work for ICC with placenta skeletal muscle?
Verified Customer
Verified customer
Asked: 2019-04-24
Answer
According to the expression profile of placenta skeletal muscle, CFL2 is highly expressed in placenta skeletal muscle. So, it is likely that anti-Cofilin 2/CFL2 antibody RP1107 will work for ICC with placenta skeletal muscle.
Boster Scientific Support
Answered: 2019-04-24
