Product Info Summary
SKU: | A12025 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | WB |
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Product info
Product Name
Anti-COBRA1 (NELF B) Antibody
SKU/Catalog Number
A12025
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-COBRA1 (NELF B) Antibody (Catalog # A12025). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-COBRA1 (NELF B) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12025)
Host
Mouse
Contents
PBS + 0.05% NaN3
Clonality
Monoclonal
Clone Number
F7E4
Isotype
IgG1
Immunogen
Recombinant full length human COBRA1 purified from E. coli. Immunogen species is Human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12025 is reactive to NELFB in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
65697 MW
Background of NELFB
COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12025 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
![A12025 Western_blot testing image 1 A12025 Western_blot testing image 1](https://www.bosterbio.com/media/catalog/product/A/1/A12025-Western_blot-testing-image-1.gif)
Click image to see more details
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~62 kDa COBRA1 protein.
Protein Target Info & Infographic
Gene/Protein Information For NELFB (Source: Uniprot.org, NCBI)
Gene Name
NELFB
Full Name
Negative elongation factor B
Weight
65697 MW
Superfamily
NELF-B family
Alternative Names
Negative elongation factor B NELFB COBRA1, NELF-B negative elongation factor complex member B negative elongation factor B|cofactor of BRCA1|negative elongation factor protein B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NELFB, check out the NELFB Infographic
![NELFB infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NELFB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-COBRA1 (NELF B) Antibody (A12025)
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No publications found for A12025
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