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Product Info Summary
| SKU: | A04936 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CNTN2/Tag1 Antibody
View all Contactin-2/TAG1 Antibodies
SKU/Catalog Number
A04936
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CNTN2/Tag1 Antibody (Catalog # A04936). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CNTN2/Tag1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04936)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CNTN2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04936 is reactive to CNTN2 in Human, Mouse
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
113393 MW
Background of Contactin-2/TAG1
In conjunction with another transmembrane protein, CNTNAP2, contributes to the organization of axonal domains at nodes of Ranvier by maintaining voltage-gated potassium channels at the juxtaparanodal region. May be involved in cell adhesion.
Bentley D.R., Nature 441:315-321(2006).
Henzel W.J., Protein Sci. 13:2819-2824(2004).
Smith R.D., J. Proteome Res. 4:2070-2080(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04936 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from NIH-3T3 cells, using CNTN2 antibody A04936.
Protein Target Info & Infographic
Gene/Protein Information For CNTN2 (Source: Uniprot.org, NCBI)
Gene Name
CNTN2
Full Name
Contactin-2
Weight
113393 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Contactin-2;Axonal glycoprotein TAG-1;Axonin-1;Transient axonal glycoprotein 1;TAX-1;CNTN2;AXT, TAG1, TAX1; CNTN2 AXT, FAME5, TAG-1, TAX, TAX1 contactin 2 contactin-2|axonal glycoprotein TAG-1|axonin-1 cell adhesion molecule|contactin 2 (axonal)|contactin 2 (transiently expressed)|transient axonal glycoprotein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CNTN2, check out the CNTN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CNTN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CNTN2/Tag1 Antibody (A04936)
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No publications found for A04936
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Customer Q&As
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13 Customer Q&As for Anti-CNTN2/Tag1 Antibody
Question
Would A04936 anti-CNTN2/Tag1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
As indicated on the product datasheet, A04936 anti-CNTN2/Tag1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-17
Question
We are currently using anti-CNTN2/Tag1 antibody A04936 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The anti-CNTN2/Tag1 antibody (A04936) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-25
Question
Does anti-CNTN2/Tag1 antibody A04936 work on goat WB with placenta?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
Our lab technicians have not validated anti-CNTN2/Tag1 antibody A04936 on goat. You can run a BLAST between goat and the immunogen sequence of anti-CNTN2/Tag1 antibody A04936 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-17
Question
I was wanting to use your anti-CNTN2/Tag1 antibody for WB for mouse brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
It shows on the product datasheet, A04936 anti-CNTN2/Tag1 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-25
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-CNTN2/Tag1 antibody A04936. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-28
Question
Would anti-CNTN2/Tag1 antibody A04936 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
According to the expression profile of brain, CNTN2 is highly expressed in brain. So, it is likely that anti-CNTN2/Tag1 antibody A04936 will work for WB with brain.
Boster Scientific Support
Answered: 2019-07-16
Question
I have a question about product A04936, anti-CNTN2/Tag1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04936 anti-CNTN2/Tag1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-02
Question
I have attached the WB image, lot number and protocol we used for brain using anti-CNTN2/Tag1 antibody A04936. Please let me know if you require anything else.
K. Evans
Verified customer
Asked: 2019-01-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-18
Question
I see that the anti-CNTN2/Tag1 antibody A04936 works with WB, what is the protocol used to produce the result images on the product page?
S. Collins
Verified customer
Asked: 2018-09-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-12
Question
Is a blocking peptide available for product anti-CNTN2/Tag1 antibody (A04936)?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
We do provide the blocking peptide for product anti-CNTN2/Tag1 antibody (A04936). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-10
Question
Do you have a BSA free version of anti-CNTN2/Tag1 antibody A04936 available?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CNTN2/Tag1 antibody A04936 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-16
Question
Is this A04936 anti-CNTN2/Tag1 antibody reactive to the isotypes of CNTN2?
G. Huang
Verified customer
Asked: 2016-09-28
Answer
The immunogen of A04936 anti-CNTN2/Tag1 antibody is Synthesized peptide derived from internal of human CNTN2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-09-28
Question
I am interested in to test anti-CNTN2/Tag1 antibody A04936 on mouse brain for research purposes, then I may be interested in using anti-CNTN2/Tag1 antibody A04936 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Brown
Verified customer
Asked: 2016-06-02
Answer
The products we sell, including anti-CNTN2/Tag1 antibody A04936, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-06-02
