Click image to see more details
-
-
-
-
-
+6
Product Info Summary
| SKU: | M00122-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00122-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody catalog # M00122-2. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00122-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BHH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cleaved PARP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00122-2 is reactive to PARP1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
113084 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00122-2 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
IHC: Rat skin tissue, Human pituitary tumor tissue, Human colon tissue, Mouse skeletal muscle - gastrocnemius tissue
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Cleaved PARP expression in Jurkat cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat skin, using the Antibody at 1:50 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:50 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human colon, using the Antibody at 1:50 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody at 1:50 dilution.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Click image to see more details
Immunofluorescent analysis of HeLa cells, using Cleaved PARP Antibody .
Protein Target Info & Infographic
Gene/Protein Information For PARP1 (Source: Uniprot.org, NCBI)
Gene Name
PARP1
Full Name
Poly [ADP-ribose] polymerase 1
Weight
113084 MW
Alternative Names
Poly [ADP-ribose] polymerase 1;PARP-1;2.4.2.30;ADP-ribosyltransferase diphtheria toxin-like 1;ARTD1;NAD (+) ADP-ribosyltransferase 1;ADPRT 1;Poly[ADP-ribose] synthase 1;PARP1;ADPRT, PPOL; PARP1 ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1 poly(ADP-ribose) polymerase 1 poly [ADP-ribose] polymerase 1|ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)|ADP-ribosyltransferase NAD(+)|ADP-ribosyltransferase diphtheria toxin-like 1|DNA ADP-ribosyltransferase PARP1|NAD(+) ADP-ribosyltransferase 1|poly (ADP-ribose) polymerase family, member 1|poly(ADP-ribose) synthetase|poly(ADP-ribosyl)transferase|poly[ADP-ribose] synthase 1|protein poly-ADP-ribosyltransferase PARP1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PARP1, check out the PARP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PARP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody (M00122-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody
Question
We are currently using anti-Cleaved PARP Rabbit Monoclonal antibody M00122-2 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
The anti-Cleaved PARP Rabbit Monoclonal antibody (M00122-2) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-20
Question
My question regards to test anti-Cleaved PARP Rabbit Monoclonal antibody M00122-2 on human leukemic t-cell for research purposes, then I may be interested in using anti-Cleaved PARP Rabbit Monoclonal antibody M00122-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The products we sell, including anti-Cleaved PARP Rabbit Monoclonal antibody M00122-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-27
Question
Do you have a BSA free version of anti-Cleaved PARP Rabbit Monoclonal antibody M00122-2 available?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cleaved PARP Rabbit Monoclonal antibody M00122-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-16
Question
We have seen staining in human colon carcinoma ovarian carcinoma. Any tips? Is anti-Cleaved PARP Rabbit Monoclonal antibody supposed to stain colon carcinoma ovarian carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
Based on literature colon carcinoma ovarian carcinoma does express PARP1. Based on Uniprot.org, PARP1 is expressed in heart left ventricle, fibroblast, brain, colon carcinoma ovarian carcinoma, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PARP1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, and Ovarian carcinoma, Pubmed ID: 17177976
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 3120710, 2824474, 2891139
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-12-10
Question
Does M00122-2 anti-Cleaved PARP Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
It shows on the product datasheet, M00122-2 anti-Cleaved PARP Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Cleaved PARP Rabbit Monoclonal antibody M00122-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-22
Question
I was wanting to use your anti-Cleaved PARP Rabbit Monoclonal antibody for IF for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
V. Mitchell
Verified customer
Asked: 2019-05-14
Answer
You can see on the product datasheet, M00122-2 anti-Cleaved PARP Rabbit Monoclonal antibody has been validated for Flow Cytometry, IP, IF, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-14
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-Cleaved PARP Rabbit Monoclonal antibody M00122-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-03-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-19
Question
We were happy with the WB result of your anti-Cleaved PARP Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma nucleus using this antibody. Is that expected? Could you tell me where is PARP1 supposed to be expressed?
D. Baker
Verified customer
Asked: 2019-01-10
Answer
Based on literature, cervix carcinoma does express PARP1. Generally PARP1 expresses in nucleus. Regarding which tissues have PARP1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, and Ovarian carcinoma, Pubmed ID: 17177976
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 3120710, 2824474, 2891139
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-01-10
Question
I am looking for using your anti-Cleaved PARP Rabbit Monoclonal antibody for protein modification process studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
Thank you for your inquiry. This M00122-2 anti-Cleaved PARP Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for Flow Cytometry, IP, IF, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-07-20
Question
Does anti-Cleaved PARP Rabbit Monoclonal antibody M00122-2 work for IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2018-04-02
Answer
According to the expression profile of leukemic t-cell, PARP1 is highly expressed in leukemic t-cell. So, it is likely that anti-Cleaved PARP Rabbit Monoclonal antibody M00122-2 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2018-04-02
Question
Is this M00122-2 anti-Cleaved PARP Rabbit Monoclonal antibody reactive to the isotypes of PARP1?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
The immunogen of M00122-2 anti-Cleaved PARP Rabbit Monoclonal antibody is A synthesized peptide derived from human Cleaved PARP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-11
Question
Is a blocking peptide available for product anti-Cleaved PARP Rabbit Monoclonal antibody (M00122-2)?
E. Edwards
Verified customer
Asked: 2016-01-05
Answer
We do provide the blocking peptide for product anti-Cleaved PARP Rabbit Monoclonal antibody (M00122-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-01-05
Question
I see that the anti-Cleaved PARP Rabbit Monoclonal antibody M00122-2 works with IF, what is the protocol used to produce the result images on the product page?
C. Johnson
Verified customer
Asked: 2015-11-06
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-11-06
Question
We ordered your anti-Cleaved PARP Rabbit Monoclonal antibody for IP on colon carcinoma ovarian carcinoma a few years ago. I am using human, and We are going to use the antibody for IF next. I was wanting to use examining colon carcinoma ovarian carcinoma as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
L. Zhao
Verified customer
Asked: 2015-10-12
Answer
I have checked the website and datasheets of our anti-Cleaved PARP Rabbit Monoclonal antibody and it appears that M00122-2 has been validated on human in both IP and IF. Thus M00122-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-10-12
Question
My question regarding product M00122-2, anti-Cleaved PARP Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Lewis
Verified customer
Asked: 2014-07-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00122-2 anti-Cleaved PARP Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-07-04
