Product Info Summary
SKU: | A00624 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Cleaved-COL1A2 (G1102) Antibody
SKU/Catalog Number
A00624
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cleaved-COL1A2 (G1102) Antibody catalog # A00624. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cleaved-COL1A2 (G1102) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00624)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the N-terminal region of human COL1A2. at AA rangle: 1020-1100
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00624 is reactive to COL1A2 in Human
Applications
A00624 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
129314 MW
Background of COL1A2
Binds and is activated by neuregulins and NTAK.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
![a00624 western blotting analysis 2 a00624 western blotting analysis 2](https://www.bosterbio.com/media/catalog/product/a/0/a00624-western-blotting-analysis-2.jpg)
Click image to see more details
Western Blot (WB) analysis of VEC cells using Cleaved-COL1A2 (G1102) Polyclonal antibody.
![a00624 western blotting analysis 1 a00624 western blotting analysis 1](https://www.bosterbio.com/media/catalog/product/a/0/a00624-western-blotting-analysis-1.jpg)
Click image to see more details
Western Blot (WB) analysis of specific cells using Cleaved-COL1A2 (G1102) Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For COL1A2 (Source: Uniprot.org, NCBI)
Gene Name
COL1A2
Full Name
Collagen alpha-2(I) chain
Weight
129314 MW
Superfamily
fibrillar collagen family
Alternative Names
alpha 2(I)-collagen; Alpha-2 type I collagen; collagen alpha-2(I) chain; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-2 chain; collagen, type I, alpha 2; OI4; osteogenesis imperfecta type IV; type I procollagen COL1A2 EDSARTH2, EDSCV, OI4 collagen type I alpha 2 chain collagen alpha-2(I) chain|alpha 2 type I procollagen|alpha 2(I) procollagen|alpha 2(I)-collagen|alpha-2 type I collagen|collagen I, alpha-2 polypeptide|collagen of skin, tendon and bone, alpha-2 chain|collagen, type I, alpha 2|epididymis secretory sperm binding protein|type I procollagen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL1A2, check out the COL1A2 Infographic
![COL1A2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL1A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cleaved-COL1A2 (G1102) Antibody (A00624)
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No publications found for A00624
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Customer Q&As
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3 Customer Q&As for Anti-Cleaved-COL1A2 (G1102) Antibody
Question
Will A00624 anti-Cleaved-COL1A2 (G1102) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Krishna
Verified customer
Asked: 2020-03-05
Answer
You can see on the product datasheet, A00624 anti-Cleaved-COL1A2 (G1102) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-05
Question
Is there a BSA free version of anti-Cleaved-COL1A2 (G1102) antibody A00624 available?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Cleaved-COL1A2 (G1102) antibody A00624 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-12
Question
Is a blocking peptide available for product anti-Cleaved-COL1A2 (G1102) antibody (A00624)?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
We do provide the blocking peptide for product anti-Cleaved-COL1A2 (G1102) antibody (A00624). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-16