Click image to see more details
Product Info Summary
| SKU: | RP1042 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Claudin 2/CLDN2 Antibody Picoband®
SKU/Catalog Number
RP1042
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Claudin 2/CLDN2 Antibody catalog # RP1042. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Claudin 2/CLDN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1042)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Claudin 2 recombinant protein (Position: A38-V230). Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1042 is reactive to CLDN2 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
24549 MW
Background of CLDN2
Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. By genomic sequence analysis, this gene is mapped to chromosome Xq22.3-q23. This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. By expression in a human intestinal epithelial cell line, it was determined that the intestine-specific homeodomain proteins CDX1 and CDX2 activated a reporter plasmid driven by the CLDN2 promoter.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1042 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human
Positive Control
WB: Rat Kidney Tissue, Rat Liver Tissue
IHC: Human Intestinal Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Claudin 2 antibody, RP1042, Western blotting
All lanes: Anti Claudin 2 (RP1042) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 25KD
Observed bind size: 25KD
Click image to see more details
Anti-Claudin 2 antibody, RP1042, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For CLDN2 (Source: Uniprot.org, NCBI)
Gene Name
CLDN2
Full Name
Claudin-2
Weight
24549 MW
Superfamily
claudin family
Alternative Names
Claudin-2;SP82;CLDN2;PSEC0059, SP82, UNQ705/PRO1356; CLDN2 claudin 2 claudin-2|SP82
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLDN2, check out the CLDN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLDN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Claudin 2/CLDN2 Antibody Picoband® (RP1042)
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Customer Q&As
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16 Customer Q&As for Anti-Claudin 2/CLDN2 Antibody Picoband®
Question
Do you have a BSA free version of anti-Claudin 2/CLDN2 antibody RP1042 available?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Claudin 2/CLDN2 antibody RP1042 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-24
Question
Does RP1042 anti-Claudin 2/CLDN2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
It shows on the product datasheet, RP1042 anti-Claudin 2/CLDN2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-27
Question
We have tried in the past anti-Claudin 2/CLDN2 antibody for WB on teratocarcinoma in a previous experiment. I am using rat, and I plan to use the antibody for IHC next. We are interested in examining teratocarcinoma as well as colon skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
N. Brown
Verified customer
Asked: 2019-12-13
Answer
I have checked the website and datasheets of our anti-Claudin 2/CLDN2 antibody and it seems that RP1042 has been validated on rat in both WB and IHC. Thus RP1042 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-13
Question
I have attached the WB image, lot number and protocol we used for teratocarcinoma using anti-Claudin 2/CLDN2 antibody RP1042. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-12
Question
My question regarding product RP1042, anti-Claudin 2/CLDN2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1042 anti-Claudin 2/CLDN2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-04
Question
I see that the anti-Claudin 2/CLDN2 antibody RP1042 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-03
Question
Our team were well pleased with the WB result of your anti-Claudin 2/CLDN2 antibody. However we have been able to see positive staining in teratocarcinoma cell junction using this antibody. Is that expected? Could you tell me where is CLDN2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
Based on literature, teratocarcinoma does express CLDN2. Generally CLDN2 expresses in cell junction, tight junction. Regarding which tissues have CLDN2 expression, here are a few articles citing expression in various tissues:
Colon epithelium, Pubmed ID: 11934881
Colon, and Skin, Pubmed ID: 15489334
Teratocarcinoma, Pubmed ID: 16303743
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-03
Question
Does anti-Claudin 2/CLDN2 antibody RP1042 work for IHC with teratocarcinoma?
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
According to the expression profile of teratocarcinoma, CLDN2 is highly expressed in teratocarcinoma. So, it is likely that anti-Claudin 2/CLDN2 antibody RP1042 will work for IHC with teratocarcinoma.
Boster Scientific Support
Answered: 2018-11-15
Question
Is a blocking peptide available for product anti-Claudin 2/CLDN2 antibody (RP1042)?
A. Kulkarni
Verified customer
Asked: 2018-07-11
Answer
We do provide the blocking peptide for product anti-Claudin 2/CLDN2 antibody (RP1042). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-07-11
Question
We are interested in to test anti-Claudin 2/CLDN2 antibody RP1042 on human teratocarcinoma for research purposes, then I may be interested in using anti-Claudin 2/CLDN2 antibody RP1042 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
The products we sell, including anti-Claudin 2/CLDN2 antibody RP1042, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-22
Question
Is this RP1042 anti-Claudin 2/CLDN2 antibody reactive to the isotypes of CLDN2?
Verified Customer
Verified customer
Asked: 2017-12-21
Answer
The immunogen of RP1042 anti-Claudin 2/CLDN2 antibody is E.coli-derived human Claudin 2 recombinant protein (Position: A38-V230). Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-21
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for teratocarcinoma using anti-Claudin 2/CLDN2 antibody RP1042. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-19
Question
We have been able to see staining in rat colon epithelium. Are there any suggestions? Is anti-Claudin 2/CLDN2 antibody supposed to stain colon epithelium positively?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
From literature colon epithelium does express CLDN2. From Uniprot.org, CLDN2 is expressed in kidney epithelium, colon epithelium, thalamus, teratocarcinoma, colon skin, among other tissues. Regarding which tissues have CLDN2 expression, here are a few articles citing expression in various tissues:
Colon epithelium, Pubmed ID: 11934881
Colon, and Skin, Pubmed ID: 15489334
Teratocarcinoma, Pubmed ID: 16303743
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-06-19
Question
I was wanting to use your anti-Claudin 2/CLDN2 antibody for IHC for human teratocarcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human teratocarcinoma identification?
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
You can see on the product datasheet, RP1042 anti-Claudin 2/CLDN2 antibody has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human teratocarcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-06
Question
We want using your anti-Claudin 2/CLDN2 antibody for tight junction interactions studies. Has this antibody been tested with western blotting on rat liver tissue? We would like to see some validation images before ordering.
S. Yang
Verified customer
Asked: 2015-08-20
Answer
Thanks for your inquiry. This RP1042 anti-Claudin 2/CLDN2 antibody is tested on rat liver tissue, kidney tissue, tissue lysate, intestinal cancer tissue. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-08-20
Question
We are currently using anti-Claudin 2/CLDN2 antibody RP1042 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on bovine tissues as well?
M. Thomas
Verified customer
Asked: 2014-05-29
Answer
The anti-Claudin 2/CLDN2 antibody (RP1042) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-05-29
