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Product Info Summary
| SKU: | M03134-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Cow, Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IP, ICC, WB |
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Product info
Product Name
Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody
View all Clathrin Heavy Chain 1/CHC17 Antibodies
SKU/Catalog Number
M03134-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (Catalog# M03134-1). Tested in Flow Cytometry, IP, WB, ICC, ELISA application(s). This antibody reacts with Rat, Cow, Mouse, Human, Pig.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03134-1)
Host
Mouse
Contents
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
BF-06
Isotype
Mouse IgM
Immunogen
The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa intracellular protein involved in receptor-mediated endocytosis.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M03134-1 is reactive to CTLC in Cow, Human, Mouse, Pig, Rat
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
191615 MW
Background of Clathrin Heavy Chain 1/CHC17
Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03134-1 is guaranteed for ELISA, Flow Cytometry, IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2-5 μg/ml; positive control: human blood leukocytes. Intracellular staining.
Validation Images & Assay Conditions
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Figure 1. Immunocytochemistry staining of CLTC using Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (M03134-1).
Immunocytochemistry staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
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Figure 2. Immunocytochemistry staining of CLTC using Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (M03134-1).
Immunocytochemistry staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Protein Target Info & Infographic
Gene/Protein Information For CTLC (Source: Uniprot.org, NCBI)
Gene Name
CTLC
Full Name
Clathrin heavy chain 1
Weight
191615 MW
Superfamily
clathrin heavy chain family
Alternative Names
CHC, CLHC, MRD56 CLTC CHC, CHC17, CLH-17L2, Hc, MRD56, CLTC clathrin heavy chain clathrin heavy chain 1|clathrin heavy chain on chromosome 17|clathrin, heavy polypeptide (Hc)|clathrin, heavy polypeptide-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTLC, check out the CTLC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTLC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Clathrin Heavy Chain Purified CLTC Monoclonal Antibody (M03134-1)
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