Anti-CIP75 UBQLN4 Antibody

Product Name

Anti-CIP75 UBQLN4 Antibody

View all UBQLN4/CIP75 Antibodies

SKU/Catalog Number

A08887-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CIP75 UBQLN4 Antibody (Catalog # A08887-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

CIP75 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CIP75 UBQLN4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08887-1)

Host

Rabbit

Contents

CIP75 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CIP75 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CIP75. The immunogen is located within the first 50 amino acids of CIP75.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

CUEDC2 antibody will not cross-react with CUEDC1.

Reactive Species

A08887-1 is reactive to UBQLN4 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

63853 MW

Background of UBQLN4/CIP75

The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A08887-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CIP75 antibody can be used for detection of CIP75 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Positive Control

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of CIP75 in 3T3 cell lysate with CIP75 antibody at (A) 1 and (B) 2 μg/mL.

Click image to see more details

Immunohistochemistry of CIP75 in mouse kidney tissue with CIP75 antibody at 5 μg/mL.

Gene/Protein Information For UBQLN4 (Source: Uniprot.org, NCBI)

Alternative Names

A1Uataxin-1 ubiquitin-like interacting protein; Ataxin-1 ubiquitin-like-interacting protein A1U; C1orf6ubiquilin-4; chromosome 1 open reading frame 6; UBINCIP75; ubiquilin 4 UBQLN4 A1U, A1Up, C1orf6, CIP75, UBIN ubiquilin 4 ubiquilin-4|ataxin-1 interacting ubiquitin-like protein|ataxin-1 ubiquitin-like interacting protein|ataxin-1 ubiquitin-like-interacting protein A1U|connexin43-interacting protein of 75 kDa

For more info on UBQLN4, check out the UBQLN4 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for UBQLN4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the UBQLN4 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis