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Product Info Summary
| SKU: | A01192-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Choline Acetyltransferase/CHAT Antibody Picoband®
View all Choline Acetyltransferase/ChAT Antibodies
SKU/Catalog Number
A01192-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Choline Acetyltransferase/CHAT Antibody Picoband® catalog # A01192-3. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Choline Acetyltransferase/CHAT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01192-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CHAT recombinant protein (Position: D446-R652). Human CHAT shares 88.4% and 87% amino acid (aa) sequence identity with mouse and rat CHAT, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A01192-3 is reactive to CHAT in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
83 kDa
Calculated molecular weight
82536 MW
Background of Choline Acetyltransferase/ChAT
Choline acetyltransferase (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01192-3 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: rat testis tissue, mouse testis tissue
IHC: mouse brain tissue, rat brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 2. IHC analysis of CHAT using anti-CHAT antibody (A01192-3).
CHAT was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CHAT Antibody (A01192-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CHAT using anti-CHAT antibody (A01192-3).
CHAT was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CHAT Antibody (A01192-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 1. Western blot analysis of CHAT using anti-CHAT antibody (A01192-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHAT antigen affinity purified polyclonal antibody (Catalog # A01192-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHAT at approximately 83KD. The expected band size for CHAT is at 83KD.
Protein Target Info & Infographic
Gene/Protein Information For CHAT (Source: Uniprot.org, NCBI)
Gene Name
CHAT
Full Name
Choline O-acetyltransferase
Weight
82536 MW
Superfamily
carnitine/choline acetyltransferase family
Alternative Names
Choline O-acetyltransferase;CHOACTase;ChAT;Choline acetylase;2.3.1.6;CHAT; CHAT CHOACTASE, CMS1A, CMS1A2, CMS6 choline O-acetyltransferase choline O-acetyltransferase|acetyl CoA:choline O-acetyltransferase|choline acetylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHAT, check out the CHAT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Choline Acetyltransferase/CHAT Antibody Picoband® (A01192-3)
Hello CJ!
A01192-3 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
PROTECTIVE EFFECTS OF ICARIIN ON COGNITIVE DEFICITS INDUCED BY CHRONIC CEREBRAL HYPOPERFUSION IN RATS
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer
Autonomic remodeling may be responsible for decreased incidence of aortic dissection in STZ-induced diabetic rats via down-regulation of matrix metalloprotease 2
Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons
Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma
Yu H, Xia H, Tang Q, Xu H, Wei G, Chen Y, Dai X, Gong Q, Bi F. Sci Rep. 2017 Jan 19;7:40802. doi: 10.1038/srep40802. Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation
Zhao D, Xu X, Pan L, Zhu W, Fu X, Guo L, Lu Q, Wang J. J Neuroinflammation. 2017 Dec 2;14(1):234. doi: 10.1186/s12974-017-1007-2. Pharmacologic activation of cholinergic alpha7 nicotinic receptors mitigates depressive-like behavior in a mouse mode...
Liu B, Deng C, Zhang Y, Zhang J. Neural Regen Res. 2012 Jul 5;7(19):1463-8. Doi: 10.3969/J.Issn.1673-5374.2012.19.003. Wnt3A Expression During The Differentiation Of Adipose-Derived Stem Cells Into Cholinergic Neurons.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Choline Acetyltransferase/CHAT Antibody Picoband®
Question
My lab would like using your anti-Choline Acetyltransferase/CHAT antibody for myasthenic syndrome studies. Has this antibody been tested with western blotting on rat testis tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We appreciate your inquiry. This A01192-3 anti-Choline Acetyltransferase/CHAT antibody is tested on rat testis tissue, mouse brain. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-25
Question
We have been able to see staining in rat placenta. What should we do? Is anti-Choline Acetyltransferase/CHAT antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
According to literature placenta does express CHAT. According to Uniprot.org, CHAT is expressed in placenta, spinal cord, lymphocyte, among other tissues. Regarding which tissues have CHAT expression, here are a few articles citing expression in various tissues:
Lymphocyte, Pubmed ID: 1784419
Placenta, Pubmed ID: 3183663
Spinal cord, Pubmed ID: 1337937
Boster Scientific Support
Answered: 2019-12-13
Question
Does anti-Choline Acetyltransferase/CHAT antibody A01192-3 work for IHC with placenta?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
According to the expression profile of placenta, CHAT is highly expressed in placenta. So, it is likely that anti-Choline Acetyltransferase/CHAT antibody A01192-3 will work for IHC with placenta.
Boster Scientific Support
Answered: 2019-09-04
Question
We ordered your anti-Choline Acetyltransferase/CHAT antibody for WB on placenta in a previous experiment. I am using human, and We intend to use the antibody for IHC next. I am looking for examining placenta as well as spinal cord in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
I looked at the website and datasheets of our anti-Choline Acetyltransferase/CHAT antibody and it seems that A01192-3 has been tested on human in both WB and IHC. Thus A01192-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-14
Question
I see that the anti-Choline Acetyltransferase/CHAT antibody A01192-3 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Choline Acetyltransferase/CHAT antibody A01192-3. Let me know if you need anything else.
M. Dhar
Verified customer
Asked: 2018-05-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-08
Question
I was wanting to use your anti-Choline Acetyltransferase/CHAT antibody for IHC for rat placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat placenta identification?
Verified Customer
Verified customer
Asked: 2018-01-08
Answer
It shows on the product datasheet, A01192-3 anti-Choline Acetyltransferase/CHAT antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-08
Question
Is this A01192-3 anti-Choline Acetyltransferase/CHAT antibody reactive to the isotypes of CHAT?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
The immunogen of A01192-3 anti-Choline Acetyltransferase/CHAT antibody is E.coli-derived human CHAT recombinant protein (Position: D446-R652). Human CHAT shares 88.4% and 87% amino acid (aa) sequence identity with mouse and rat CHAT, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-12
Question
I have a question about product A01192-3, anti-Choline Acetyltransferase/CHAT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Carter
Verified customer
Asked: 2017-01-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01192-3 anti-Choline Acetyltransferase/CHAT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-12
Question
We are currently using anti-Choline Acetyltransferase/CHAT antibody A01192-3 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
C. Roberts
Verified customer
Asked: 2016-09-22
Answer
The anti-Choline Acetyltransferase/CHAT antibody (A01192-3) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-22
Question
Do you have a BSA free version of anti-Choline Acetyltransferase/CHAT antibody A01192-3 available?
K. Yang
Verified customer
Asked: 2016-07-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Choline Acetyltransferase/CHAT antibody A01192-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-07-08
Question
Would A01192-3 anti-Choline Acetyltransferase/CHAT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Taylor
Verified customer
Asked: 2015-12-28
Answer
You can see on the product datasheet, A01192-3 anti-Choline Acetyltransferase/CHAT antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-12-28
Question
I am interested in to test anti-Choline Acetyltransferase/CHAT antibody A01192-3 on rat placenta for research purposes, then I may be interested in using anti-Choline Acetyltransferase/CHAT antibody A01192-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Baker
Verified customer
Asked: 2015-04-21
Answer
The products we sell, including anti-Choline Acetyltransferase/CHAT antibody A01192-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-04-21
Question
Is a blocking peptide available for product anti-Choline Acetyltransferase/CHAT antibody (A01192-3)?
D. Parker
Verified customer
Asked: 2015-01-13
Answer
We do provide the blocking peptide for product anti-Choline Acetyltransferase/CHAT antibody (A01192-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-01-13
Question
See below the WB image, lot number and protocol we used for placenta using anti-Choline Acetyltransferase/CHAT antibody A01192-3. Please let me know if you require anything else.
J. Williams
Verified customer
Asked: 2013-07-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-07-11
