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Product Info Summary
| SKU: | A02868-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-CES2 Antibody (Center)
View all Carboxylesterase 2/CES2 Antibodies
SKU/Catalog Number
A02868-1
Size
80 µl
Description
Boster Bio Anti-CES2 Antibody (Center) (Catalog # A02868-1). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CES2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02868-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CES2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 340-369 amino acids from the Central region of human CES2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02868-1 is reactive to CES2 in Human
Reconstitution
Calculated molecular weight
61807 Da
Background of Carboxylesterase 2/CES2
CES2 is a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02868-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:50-1:100
FC: 1:25
Validation Images & Assay Conditions
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CES2 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane). This demonstrates the CES2 antibody detected the CES2 protein (arrow).
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All lanes : Anti-CES2 Antibody (Center) at 1:2000 dilution
Lane 1: SW620 whole cell lysates
Lane 2: human liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 62 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-CES2 Antibody (Center) at 1:2000 dilution
Lane 1: HepG2 whole cell lysates
Lane 2: human liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 62 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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CES2 antibody (Center) (Cat. #A02868-1) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CES2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Overlay histogram showing U-87 MG cells stained with A02868-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A02868-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Overlay histogram showing MCF-7 cells stained with A02868-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A02868-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For CES2 (Source: Uniprot.org, NCBI)
Gene Name
CES2
Full Name
Cocaine esterase
Weight
61807 Da
Superfamily
type-B carboxylesterase/lipase family
Alternative Names
Cocaine esterase, Carboxylesterase 2, CE-2, hCE-2, Methylumbelliferyl-acetate deacetylase 2, CES2, ICE CES2 CE-2A1, PCE-2, iCE, CES2 carboxylesterase 2 cocaine esterase|carboxylesterase 2 (intestine, liver)|intestinal carboxylesterase; liver carboxylesterase-2|methylumbelliferyl-acetate deacetylase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CES2, check out the CES2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CES2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CES2 Antibody (Center) (A02868-1)
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