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Product Info Summary
| SKU: | A03954-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CEP55 Antibody Picoband®
SKU/Catalog Number
A03954-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CEP55 Antibody Picoband® catalog # A03954-4. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CEP55 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03954-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human CEP55 recombinant protein (Position: E115-K464). Human CEP55 shares 76.3% and 78% amino acid (aa) sequence identity with mouse and rat CEP55, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03954-4 is reactive to CEP55 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated molecular weight
54.178kDa
Background of CEP55
Centrosomal protein of 55 kDa (Cep55), is a protein that in humans is encoded by the CEP55 gene. Enables identical protein binding activity. Involved in cranial skeletal system development; establishment of protein localization; and midbody abscission. Acts upstream of or within mitotic cytokinesis. Located in Flemming body and centrosome. Implicated in multinucleated neurons, anhydramnios, renal dysplasia, cerebellar hypoplasia and hydranencephaly.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03954-4 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CEP55 using anti-CEP55 antibody (A03954-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP55 antigen affinity purified polyclonal antibody (A03954-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP55 at approximately 54 kDa. The expected band size for CEP55 is at 54 kDa.
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Figure 2. Flow Cytometry analysis of A549 cells using anti-CEP55 antibody (A03954-4).
Overlay histogram showing A549 cells stained with A03954-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEP55 Antibody (A03954-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CEP55 (Source: Uniprot.org, NCBI)
Gene Name
CEP55
Full Name
Centrosomal protein of 55 kDa
Weight
54.178kDa
Alternative Names
C10orf3; cancer/testis antigen 111; centrosomal protein 55kDa; centrosomal protein of 55 kDa; Cep55; chromosome 10 open reading frame 3; CT111; FLJ10540; Up-regulated in colon cancer 6; URCC6 CEP55 C10orf3, CT111, MARCH, URCC6 centrosomal protein 55 centrosomal protein of 55 kDa|cancer/testis 111|centrosomal protein 55kDa|up-regulated in colon cancer 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEP55, check out the CEP55 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEP55: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CEP55 Antibody Picoband® (A03954-4)
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