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Product Info Summary
| SKU: | A06361-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CEP135 Antibody Picoband®
SKU/Catalog Number
A06361-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CEP135 Antibody Picoband® catalog # A06361-4. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CEP135 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06361-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human CEP135 recombinant protein (Position: E28-E1034). Human CEP135 shares 82.8% amino acid (aa) sequence identity with mouse CEP135.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06361-4 is reactive to CEP135 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated molecular weight
133.49kDa
Background of CEP135
Centrosomal protein of 135 kDa is a protein that in humans is encoded by the CEP135 gene. This gene encodes a centrosomal protein, which acts as a scaffolding protein during early centriole biogenesis, and is also required for centriole-centriole cohesion during interphase. Mutations in this gene are associated with autosomal recessive primary microcephaly-8.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06361-4 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CEP135 using anti-CEP135 antibody (A06361-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP135 antigen affinity purified polyclonal antibody (A06361-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP135 at approximately 133 kDa. The expected band size for CEP135 is at 133 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CEP135 (Source: Uniprot.org, NCBI)
Gene Name
CEP135
Full Name
Centrosomal protein of 135 kDa
Weight
133.49kDa
Superfamily
CEP135/TSGA10 family
Alternative Names
Centrosomal protein of 135 kDa CEP135 CEP4, KIAA0635, MCPH8 centrosomal protein 135 centrosomal protein of 135 kDa|centrosomal protein 135kDa|centrosomal protein 4|centrosome protein cep135
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEP135, check out the CEP135 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEP135: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CEP135 Antibody Picoband® (A06361-4)
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