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Product Info Summary
| SKU: | M00386 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-CEBP alpha Rabbit Monoclonal Antibody
View all CEBP alpha Antibodies
SKU/Catalog Number
M00386
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CEBP alpha Rabbit Monoclonal Antibody catalog # M00386. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CEBP alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00386)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FIE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CEBP alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00386 is reactive to CEBPA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
125 kDa
Calculated molecular weight
37561 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00386 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IP 1:50
FC 1:50
Positive Control
WB: U937 cell
Validation Images & Assay Conditions
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Western blot analysis of CEBP alpha expression in U937 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CEBPA (Source: Uniprot.org, NCBI)
Gene Name
CEBPA
Full Name
CCAAT/enhancer-binding protein alpha
Weight
37561 MW
Superfamily
bZIP family
Alternative Names
CCAAT/enhancer-binding protein alpha ;C/EBP alpha ;CEBPA ;CEBP ; CEBPA C/EBP-alpha, CEBP CCAAT enhancer binding protein alpha CCAAT/enhancer-binding protein alpha|CCAAT/enhancer binding protein (C/EBP), alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEBPA, check out the CEBPA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEBPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CEBP alpha Rabbit Monoclonal Antibody (M00386)
Hello CJ!
M00386 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen G,Wang Q,Li Z,Yang Q,Liu Y,Du Z,Zhang G,Song Y.Circular RNA CDR1as promotes adipogenic and suppresses osteogenic differentiation of BMSCs in steroid-induced osteonecrosis of the femoral head.Bone.2020 Apr;133:115258.doi:10.1016/j.bone.2020.115258.Epu
Species: Human
M00386 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:NA
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Customer Q&As
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4 Customer Q&As for Anti-CEBP alpha Rabbit Monoclonal Antibody
Question
We are currently using anti-CEBP alpha Rabbit Monoclonal antibody M00386 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The anti-CEBP alpha Rabbit Monoclonal antibody (M00386) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-06
Question
We were content with the WB result of your anti-CEBP alpha Rabbit Monoclonal antibody. However we have observed positive staining in adipose tissue of abdominal region nucleolus using this antibody. Is that expected? Could you tell me where is CEBPA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
According to literature, adipose tissue of abdominal region does express CEBPA. Generally CEBPA expresses in nucleus., isoform 4: nucleus, nucleolus. Regarding which tissues have CEBPA expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15057824
Pancreas, Pubmed ID: 15489334
Umbilical cord, Pubmed ID: 7575576
Boster Scientific Support
Answered: 2017-08-07
Question
We have seen staining in human umbilical cord. Any tips? Is anti-CEBP alpha Rabbit Monoclonal antibody supposed to stain umbilical cord positively?
N. Patel
Verified customer
Asked: 2014-10-03
Answer
Based on literature umbilical cord does express CEBPA. Based on Uniprot.org, CEBPA is expressed in adipose tissue of abdominal region, umbilical cord, liver, pancreas, among other tissues. Regarding which tissues have CEBPA expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15057824
Pancreas, Pubmed ID: 15489334
Umbilical cord, Pubmed ID: 7575576
Boster Scientific Support
Answered: 2014-10-03
Question
Our lab used your anti-CEBP alpha Rabbit Monoclonal antibody for IP on umbilical cord in a previous experiment. I am using mouse, and We are going to use the antibody for WB next. We need examining umbilical cord as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for WB?
J. Patel
Verified customer
Asked: 2013-06-13
Answer
I took a look at the website and datasheets of our anti-CEBP alpha Rabbit Monoclonal antibody and it appears that M00386 has been validated on mouse in both IP and WB. Thus M00386 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-06-13
