Anti-CDw75/ST6GAL1 Antibody Picoband™

Product Name

Anti-CDw75/ST6GAL1 Antibody Picoband™

View all ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibodies

SKU/Catalog Number

A03156-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDw75/ST6GAL1 Antibody Picoband™ catalog # A03156-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CDw75/ST6GAL1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03156-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CDw75/ST6GAL1 recombinant protein (Position: M1-C406).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03156-1 is reactive to ST6GAL1 in Human

Applications

A03156-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

47 kDa

Calculated molecular weight

65411 MW

Background of ST6 Gal Sialyltransferase 1/ST6GAL1/CD75

Beta-galactoside alpha-2,6-sialyltransferase 1 is an enzyme that in humans is encoded by the ST6GAL1 gene. This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CDw75/ST6GAL1 using anti-CDw75/ST6GAL1 antibody (A03156-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDw75/ST6GAL1 antigen affinity purified polyclonal antibody (Catalog # A03156-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDw75/ST6GAL1 at approximately 47 kDa. The expected band size for CDw75/ST6GAL1 is at 47 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-CDw75/ST6GAL1 antibody (A03156-1).
Overlay histogram showing HepG2 cells stained with A03156-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDw75/ST6GAL1 Antibody (A03156-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For ST6GAL1 (Source: Uniprot.org, NCBI)

Alternative Names

Alpha 2,6-ST; B-cell antigen CD75; beta-galactoside alpha-2,6-sialyltransferase 1; CD75; CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; EC 2.4.99.1; MGC48859; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase); Sialyltransferase 1; SIAT1; SIAT1Alpha 2,6-ST 1; ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; ST6Gal I; ST6GAL1; ST6GalI; ST6N ST6GAL1 SIAT1, ST6GalI, ST6N ST6 beta-galactoside alpha-2,6-sialyltransferase 1 beta-galactoside alpha-2,6-sialyltransferase 1|B-cell antigen CD75|CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase|CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1|ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1|ST6 beta-galactosamide alpha-2,6-sialyltranferase 1|ST6Gal I|alpha 2,6-ST 1|sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase)

For more info on ST6GAL1, check out the ST6GAL1 Infographic

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In this infographic, you will see the following information for ST6GAL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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