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Product Info Summary
| SKU: | A05786-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CDKN2AIP/CARF Antibody Picoband®
SKU/Catalog Number
A05786-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CDKN2AIP-CARF Antibody Picoband® catalog # A05786-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CDKN2AIP/CARF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05786-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived mouse CDKN2AIP/CARF recombinant protein (Position: R183-T689). Human CDKN2AIP/CARF shares 78.3% and 88.1% amino acid (aa) sequence identity with human and rat CDKN2AIP/CARF, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05786-1 is reactive to Carf in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated molecular weight
80.698kDa
Background of ALS2CR8
Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in positive regulation of transcription by RNA polymerase II. Acts upstream of or within regulation of DNA-binding transcription factor activity. Located in granular component. Is expressed in several structures, including central nervous system; eye; genitourinary system; gut; and skeletal muscle tissue. Orthologous to human CARF (calcium responsive transcription factor).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05786-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDKN2AIP/CARF using anti-CDKN2AIP/CARF antibody (A05786-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDKN2AIP/CARF antigen affinity purified polyclonal antibody (A05786-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDKN2AIP/CARF at approximately 75 kDa. The expected band size for CDKN2AIP/CARF is at 61 kDa.
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Figure 2. Flow Cytometry analysis of NIH/3T3 cells using anti-CDKN2AIP/CARF antibody (A05786-1).
Overlay histogram showing NIH/3T3 cells stained with A05786-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDKN2AIP/CARF Antibody (A05786-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Carf (Source: Uniprot.org, NCBI)
Gene Name
Carf
Full Name
Calcium-responsive transcription factor
Weight
80.698kDa
Alternative Names
amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8; amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein; Calcium-response factor; CaRF; CARFDKFZp667N246; FLJ12675; FLJ21579; NYD-SP24; Testis development protein NYD-SP24 CARF ALS2CR8, NYD-SP24 calcium responsive transcription factor calcium-responsive transcription factor|amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8|amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein|calcium-response factor|testis development protein NYD-SP24
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Carf, check out the Carf Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Carf: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDKN2AIP/CARF Antibody Picoband® (A05786-1)
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