Product Info Summary
SKU: | A13717-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-CDCA4 Antibody
SKU/Catalog Number
A13717-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CDCA4 Antibody (Catalog# A13717-1). Tested in IHC-P, IF, ICC, WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDCA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13717-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CDCA4. Immunogen sequence location: 121-170
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13717-1 is reactive to CDCA4 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
26114 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13717-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500-2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CDCA4 Antibody. The picture on the right is blocked with the synthesized peptide.
Click image to see more details
Immunofluorescence analysis of HepG2 cells, using CDCA4 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CDCA4 (Source: Uniprot.org, NCBI)
Gene Name
CDCA4
Full Name
Cell division cycle-associated protein 4
Weight
26114 MW
Alternative Names
CDCA4;HEPP;Cell division cycle-associated protein 4;Hematopoietic progenitor protein CDCA4 HEPP, SEI-3/HEPP cell division cycle associated 4 cell division cycle-associated protein 4|hematopoietic progenitor protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDCA4, check out the CDCA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDCA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDCA4 Antibody (A13717-1)
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No publications found for A13717-1
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CDCA4 Antibody
Question
Is a blocking peptide available for product anti-CDCA4 antibody (A13717-1)?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
We do provide the blocking peptide for product anti-CDCA4 antibody (A13717-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-21
Question
I am looking for to test anti-CDCA4 antibody A13717-1 on human female gonad for research purposes, then I may be interested in using anti-CDCA4 antibody A13717-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
The products we sell, including anti-CDCA4 antibody A13717-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-11
Question
We are currently using anti-CDCA4 antibody A13717-1 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on horse tissues as well?
P. Edwards
Verified customer
Asked: 2019-12-27
Answer
The anti-CDCA4 antibody (A13717-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-27
Question
I see that the anti-CDCA4 antibody A13717-1 works with IF, what is the protocol used to produce the result images on the product page?
R. Lewis
Verified customer
Asked: 2013-09-12
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-09-12
Question
Can you help my question with product A13717-1, anti-CDCA4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Li
Verified customer
Asked: 2013-09-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13717-1 anti-CDCA4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-09-05