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Product Info Summary
| SKU: | M10074 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-CDCA3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M10074
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDCA3 Rabbit Monoclonal Antibody catalog # M10074. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDCA3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10074)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IAF-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDCA3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M10074 is reactive to CDCA3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
29 kDa
Calculated molecular weight
29 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M10074 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
ICC/IF 1:50-1:100
Positive Control
WB: human MCF-7 whole cell, human HepG2 whole cell, human Hela whole cell, rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDCA3 using anti-CDCA3 antibody (M10074).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDCA3 antigen affinity purified monoclonal antibody (Catalog # M10074) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDCA3 at approximately 29 kDa. The expected band size for CDCA3 is at 29 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CDCA3 (Source: Uniprot.org, NCBI)
Gene Name
CDCA3
Full Name
Cell division cycle-associated protein 3
Weight
29 kDa
Alternative Names
Cell division cycle-associated protein 3;Gene-rich cluster protein C8;Trigger of mitotic entry protein 1;TOME-1;CDCA3;C8, GRCC8, TOME1;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDCA3, check out the CDCA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDCA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDCA3 Rabbit Monoclonal Antibody (M10074)
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No publications found for M10074
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1 Customer Q&As for Anti-CDCA3 Rabbit Monoclonal Antibody
Question
We are currently using anti-CDCA3 Rabbit Monoclonal antibody M10074 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
The anti-CDCA3 Rabbit Monoclonal antibody (M10074) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-06
