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Product Info Summary
| SKU: | M01202 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD9 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01202
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD9 Rabbit Monoclonal Antibody catalog # M01202. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01202)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CED-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD9
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01202 is reactive to CD9 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
25 kDa
Calculated molecular weight
25416 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01202 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:100-1:500
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
IHC: human tonsil tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD9 using anti-CD9 antibody (M01202).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A375 whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD9 antigen affinity purified monoclonal antibody (Catalog # M01202) at 1:100 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD9 at approximately 25 kDa. The expected band size for CD9 is at 25 kDa.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using CD9 Antibody.
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Immunofluorescent analysis of Hela cells, using CD9 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For CD9 (Source: Uniprot.org, NCBI)
Gene Name
CD9
Full Name
CD9 antigen
Weight
25416 MW
Superfamily
tetraspanin (TM4SF) family
Alternative Names
CD9 antigen;5H9 antigen;Cell growth-inhibiting gene 2 protein;Leukocyte antigen MIC3;Motility-related protein;MRP-1;Tetraspanin-29;Tspan-29;p24;CD9;CD9;MIC3, TSPAN29;GIG2; CD9 BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29 CD9 molecule CD9 |5H9 |BA-2/p24 |CD9 (p24)| CD9|cell growth-inhibiting gene 2 protein|leukocyte MIC3|motility related protein-1|tetraspanin-29
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD9, check out the CD9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD9 Rabbit Monoclonal Antibody (M01202)
Hello CJ!
M01202 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The bone marrow microenvironment at single-cell resolution
Species: Mouse
Wang D,Hao C,Zhang L,Zhang J,Liu S,Li Y,Qu Y,Zhao Y,Huang R,Wei J,Yao W.Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation.Ecotoxicol Environ Saf.2020 Apr 1;192:110253.doi:10.1016/j.ecoenv.2020.110253.Epub
Species: Human,Mouse
M01202 usage in article: APP:WB, SAMPLE:FIBROBLAST, DILUTION:NA
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Customer Q&As
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5 Customer Q&As for Anti-CD9 Rabbit Monoclonal Antibody
Question
We are interested in using your anti-CD9 Rabbit Monoclonal antibody for negative regulation of platelet aggregation studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
Thank you for your inquiry. This M01202 anti-CD9 Rabbit Monoclonal antibody is tested on hela cells. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-31
Question
We ordered your anti-CD9 Rabbit Monoclonal antibody for Flow Cytometry on platelet in a previous project. I am using rat, and We want to use the antibody for WB next. Our lab want to know about examining platelet as well as trigeminal ganglion in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
I looked at the website and datasheets of our anti-CD9 Rabbit Monoclonal antibody and it seems that M01202 has been tested on rat in both Flow Cytometry and WB. Thus M01202 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-27
Question
Our lab were satisfied with the WB result of your anti-CD9 Rabbit Monoclonal antibody. However we have seen positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD9 supposed to be expressed?
M. Jha
Verified customer
Asked: 2015-02-20
Answer
From literature, leukocyte does express CD9. Generally CD9 expresses in cell membrane. Regarding which tissues have CD9 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 8486348
Ovary, Pubmed ID: 15489334
Platelet, Pubmed ID: 2358073
Boster Scientific Support
Answered: 2015-02-20
Question
We are currently using anti-CD9 Rabbit Monoclonal antibody M01202 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
K. Yang
Verified customer
Asked: 2014-09-29
Answer
The anti-CD9 Rabbit Monoclonal antibody (M01202) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-29
Question
We have seen staining in human leukocyte. What should we do? Is anti-CD9 Rabbit Monoclonal antibody supposed to stain leukocyte positively?
A. Jackson
Verified customer
Asked: 2013-04-15
Answer
Based on literature leukocyte does express CD9. Based on Uniprot.org, CD9 is expressed in trigeminal ganglion, leukocyte, ovary, platelet, among other tissues. Regarding which tissues have CD9 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 8486348
Ovary, Pubmed ID: 15489334
Platelet, Pubmed ID: 2358073
Boster Scientific Support
Answered: 2013-04-15
